Cristin-resultat-ID: 2052471
Sist endret: 2. november 2022, 15:12
NVI-rapporteringsår: 2022
Resultat
Vitenskapelig artikkel
2022

Preparation of stable polymer-liposome complexes by a novel approach employing a one-pot method

Bidragsytere:
  • Joseph Azumah
  • Gro Smistad og
  • Marianne Hiorth

Tidsskrift

Colloids and Surfaces A: Physicochemical and Engineering Aspects
ISSN 0927-7757
e-ISSN 1873-4359
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2022
Publisert online: 2022
Trykket: 2022
Volum: 653

Importkilder

Scopus-ID: 2-s2.0-85136084163

Beskrivelse Beskrivelse

Tittel

Preparation of stable polymer-liposome complexes by a novel approach employing a one-pot method

Sammendrag

The purpose of this study was to prepare stable polymer-liposome complexes using a new, improved one-pot method. Complexes prepared by the conventional method were used as reference. The one-pot method combines the vesicle formation and complexation with the polymer in a single step allowing for fewer steps compared to the conventional thin-film method followed by coating. This has the possibility of complexing the polymer on the surface and within the aqueous core of the liposomes. Soya phosphatidylcholine (SoyPC) in combination with different amounts of either dioleoyl trimethylammoniumpropane (DOTAP, positively charged) or egg phosphatidylglycerol (Egg PG, negatively charged) were complexed with alginate or chitosan, respectively. All the alginate-liposome complexes were larger compared to the naked liposomes and the zeta potential changed from positive to negative after complexation with the polymer. Polydispersity index (PDI) analysis showed narrow size distributions implying homogenous populations. The best stability was obtained by employing 20 mol% DOTAP in the membrane. This formulation was stable during the whole four months period when the stability test was performed. The chitosan-liposome complexes were generally difficult to extrude and were larger than the alginate-liposome complexes due to the need for a larger pore size in the membrane during extrusion. Based on these results, the one-pot method can be used for preparing stable alginate-liposome complexes. For the chitosan-liposome complexes alternative methods for size reduction should be investigated.

Bidragsytere

Joseph Azumah

  • Tilknyttet:
    Forfatter
    ved Seksjon for galenisk farmasi og samfunns ved Universitetet i Oslo

Gro Smistad

  • Tilknyttet:
    Forfatter
    ved Seksjon for galenisk farmasi og samfunns ved Universitetet i Oslo

Marianne Hiorth

  • Tilknyttet:
    Forfatter
    ved Seksjon for galenisk farmasi og samfunns ved Universitetet i Oslo
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