Cristin-resultat-ID: 245352
Sist endret: 11. januar 2010 13:49
Resultat
Vitenskapelig foredrag
2009

Metabolomics to elucidate the effect of antioxidant-rich diet in inflammation and oxidative stress related diseases in transgenic mice and human intervention studies

Bidragsytere:
  • Amrit Kaur Sakhi og
  • Rune Blomhoff

Workshop

Tittel: Mass Spectrometry Metabolomics Workshop

Sted:

Copenhagen
Danmark
Utbredelsesområde: Internasjonalt
Årstall: 2009
Dato fra: 24. februar 2009
Dato til: 27. februar 2009

Arrangør:

Sysdiet, NuGO and Norden

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2009

Beskrivelse Beskrivelse

Tittel

Metabolomics to elucidate the effect of antioxidant-rich diet in inflammation and oxidative stress related diseases in transgenic mice and human intervention studies

Sammendrag

Background: The main aim of the research group of professor Rune Blomhoff is to elucidate mechanisms behind protective effects of fruits and vegetable intake in inflammation and oxidative stress related diseases like cancer, cardiovascular disease, cataract and other neurodegenerative diseases. In order to study these mechanisms, we have systematically done various approaches: 1. Identification of plants that are rich in antioxidants. 2. Developed cells and transgenic mice using the reporter luciferase controlled by small oxidative stress- or inflammation related response elements. Studying the effect of antioxidant-rich plant foods on these response elements. 3. Human intervention studies with different groups of participants in order to study the effect of antioxidant-rich plant diet on different antioxidant and oxidative stress biomarkers. Here are some of the intervention studies we already have conducted. • Head and neck cancer patients (samples are collected before and after radiotherapy and studied association of plasma antioxidants and oxidative stress biomarkers with survival) • Top athlete study (samples are collected before and after intervention with blueberry/grape juice in elite athletes competing in endurance sport) • Oslo antioxidant study (samples are collected before and after intervention with antioxidant rich foods in middle-aged smoking men) • Prostate cancer study (patients with prostate cancer is treated with dietary antioxidants before surgery or radiation) • Follicular lymphoma study (patients with follicular lymphoma are studies with different oxidative stress and inflammation biomarkers) 4. Gene-array analysis in some of above mentioned human intervention studies. Methods and results: We have collected plasma samples and in some studies red blood samples from participants. The plasma samples are collected in different anticoagulant tubes (heparin, EDTA and citrate) and the samples are frozen in -80 ºC. We have analysed some antioxidant biomarkers like GSH, GSSG, total thiols (cysteine, homocysteine, cysteinyl glycine), carotenoids (lutein, zeaxanthine, -cryptoxanthine, -carotene, -carotene and lycopene), tocpherols (, -, -, and -), vitamin C (both reduced and oxidized forms) and total anatioxidant capacity measured by e.g. by FRAP or ORAC. Among oxidative stress biomarkers, we have analyzed 8-iso PGF2 and d-ROMs (total hydroperoxides). We have also analyzed cytokines in some of the human intervention studies. Future plans: After establishing gene-array analysis in some of these studies, we want to further analyse metabolomics such that we can study the different processes/pathways from gene-array analysis results in a more complete manner. We have a LC-MS-MS at our lab that we are planning to use for this purpose. From the transgenic mice, we have also different tissue samples that are frozen in -80 ºC. Samples from these animals are collected almost continuously such that sample collection procedures can be modified if required.

Bidragsytere

Amrit Kaur Sakhi

  • Tilknyttet:
    Forfatter
    ved Avdeling for ernæringsvitenskap ved Universitetet i Oslo

Rune Blomhoff

  • Tilknyttet:
    Forfatter
    ved Avdeling for ernæringsvitenskap ved Universitetet i Oslo
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