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Cristin-resultat-ID:
337188
Sist endret:
11. desember 2008, 15:01
NVI-rapporteringsår:
2008
Resultat
Vitenskapelig artikkel
2008
Correlation of Hsp70-1 and Hsp70-2 gene expression with the degree of graft-versus-host reaction in a rat skin explant model
P Novota
L Sviland
Severin Zinöcker
P Stocki
Y Balavarca
H Bickeboller
mfl.
Tidsskrift
Tidsskrift
Transplantation
ISSN 0041-1337
e-ISSN 1534-6080
NVI-nivå 1
Finn i kanalregisteret
Om resultatet
Om resultatet
Vitenskapelig artikkel
Publiseringsår: 2008
Volum: 85
Hefte: 12
Sider: 1809 - 1812
Lenker
Lenker
original online (doi)
https://doi.org/10.1097/TP.0b013e31817753f7
Importkilder
Importkilder
Scopus-ID: 2-s2.0-49149102471
Isi-ID: 000257282200020
Beskrivelse
Beskrivelse
Engelsk
Tittel
Correlation of Hsp70-1 and Hsp70-2 gene expression with the degree of graft-versus-host reaction in a rat skin explant model
Sammendrag
BACKGROUND: Graft-versus-host disease (GVHD) is the most serious complication after allogeneic hematopoietic stem-cell transplantation. A human skin explant assay has been used to predict the risk of GVHD in patients by histological grading of graft-versus-host reactions (GVHR). New molecular markers of GVHR might help to further increase the predictive value of the assay. METHODS: A rat skin explant assay has been developed to further aid in identifying potential novel molecular markers. RESULTS: In inbred rat strains GVHR were observed in skin explants co-cultured with allogeneic lymphocytes stimulated against minor or major histocompatibility antigens. The histological signs of GVHR were similar to those observed in human skin explant assays and acute GVHD lesions occurring in rats after experimental bone marrow transplantation. Heat shock protein (HSP) 70 has been shown to be expressed during GVHR. We therefore investigated the expression of the three major histocompatibility complex (MHC)-linked HSP70 genes in rat skin explants. The two major stress-inducible genes Hsp70-1 and Hsp70-2 were found to be upregulated in the allogeneic rat skin explant assays. The increase in mRNA correlated with the GVHR grade (I-IV). Interestingly, the expression of the third MHC-linked Hsp70 gene Hsp70-3 was not found to be augmented during GVHR. CONCLUSION: The observed induction of the MHC-encoded Hsp70-1 and Hsp70-2 genes might serve as new markers of GVHR and as potentially novel diagnostic tools for GVHD.
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Bidragsytere
Bidragsytere
P Novota
Forfatter
L Sviland
Forfatter
ved Helse Bergen HF - Haukeland universitetssykehus
Severin Zinöcker
Forfatter
ved Avdeling for anatomi ved Universitetet i Oslo
P Stocki
Forfatter
Y Balavarca
Forfatter
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