Cristin-resultat-ID: 344478
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
1997

Vaccine induced IgG antibodies directed to a linear epitope on the PorB protein can promote opsonophagocytosis Neisseria meningitidis by human neutrophils

Bidragsytere:
  • A Delvig
  • Terje Einar Michaelsen
  • Audun Aase
  • Ernst Arne Høiby og
  • Einar Rosenqvist

Tidsskrift

Clinical Immunology and Immunopathology
ISSN 0090-1229
e-ISSN 1090-2341
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 1997
Volum: 84
Sider: 27 - 35

Beskrivelse Beskrivelse

Tittel

Vaccine induced IgG antibodies directed to a linear epitope on the PorB protein can promote opsonophagocytosis Neisseria meningitidis by human neutrophils

Sammendrag

The serotype 15 PorB protein of Neisseria meningitidis contains an N-terminal linear immunodominant B-cell epitope located on the putative loop 1 (VR1) region. This epitope has previously been shown to stimulate antibody formation in 74% of the vaccinees after three doses of the Norwegian group B outer-membrane vesicle (OMV) vaccine. In the present study, the purified PorB protein and the 23mer synthetic peptide D63b2 covering VR1 region were immobilized onto N-hydroxysuccinimide-activated matrix and used for affinity purification of the specific IgG antibodies from sera of three selected vaccinees. PorB- and peptide D63b2-specific IgG preparations bound to the PorB protein on immunoblots and reacted with strain 44/76 and OMV complexes expressing the serotype 15 PorB protein, but not with the PorB-deficient mutant, suggesting high specificity for the PorB protein. Both PorB- and peptide D63b2-specific IgG were marginally bactericidal, but enabled strong opsonophagocytosis measured as respiratory burst response of human neutrophils and internalization of opsonized FTTC-labeled meningococci. The data indicate that about 30-57% of the bulk serum opsonic activity for the 44/76 bacteria could be ascribed to linear epitope-specific IgG1, thus contributing to vaccine-induced protection against systemic meningococcal disease via the opsonophagocytic route of pathogen clearance

Bidragsytere

A Delvig

  • Tilknyttet:
    Forfatter

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Ernst Arne Høiby

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Einar Ivanovitsj Rosenqvist

Bidragsyterens navn vises på dette resultatet som Einar Rosenqvist
  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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