Cristin-resultat-ID: 344722
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
2000

Functional activities and immunoglobulin variable regions of human and mouse monoclonal antibodies specific for the P1.7 PorA protein loop of Neisseria meningitidis

Bidragsytere:
  • Jianfu Wang
  • GA Jarvis
  • M Achtman
  • Einar Rosenqvist
  • Terje Einar Michaelsen
  • Audun Aase
  • mfl.

Tidsskrift

Infection and Immunity
ISSN 0019-9567
e-ISSN 1098-5522
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2000
Volum: 68
Sider: 1871 - 1878

Beskrivelse Beskrivelse

Tittel

Functional activities and immunoglobulin variable regions of human and mouse monoclonal antibodies specific for the P1.7 PorA protein loop of Neisseria meningitidis

Sammendrag

The meningococcal PorA protein is considered a promising vaccine candidate. Although much is understood regarding the structure of PorA proteins, little is known about the structure-function relationships of PorA antibodies. The aim of this study was to compare the functional and molecular characteristics of a human monoclonal antibody (MAb) and three murine MAbs specific for the PorA P1.7 serosubtype. Murine MAbs 207,B-4 (immunoglobulin G2a [IgG2a]) and MN14C11.6 (IgG2a) were both bactericidal and opsonophagocytic for P1.7-expressing meningococci, whereas human MAb SS269 (IgG3) and murine MAb 208,D-5 (IgA) initiated neither effector function. Epitope mapping with synthetic peptides revealed that MAbs 207,B-4 and 208,D-5 recognized the sequence ASGQ, which is the same specificity motif that a previous study had established for SS269 and MN14C11.6. Nucleotide and amino acid sequence analyses of the variable regions of the four MAbs showed that the SS269 V(H) region belonged to the VH3 family and was approximately 70% homologous to those of the murine MAbs which were all from the 7183 family, whereas the SS269 V(L) region belonged to the Vlambda1-b family and was less than 40% homologous to those of the murine MAbs which were all members of the Vkappa1 family. The Fab fragment of SS269 was cloned and expressed in Escherichia coli and was shown by enzyme-linked immunosorbent assay analyses to bind as well as intact SS269 MAb to P1.7,16 serosubtype group B strain 44/76. We conclude that distinct differences exist in the effector function activities and variable region gene sequences of human and murine P1.7-specific MAbs despite their recognition of similar epitopes

Bidragsytere

Jianfu Wang

  • Tilknyttet:
    Forfatter

GA Jarvis

  • Tilknyttet:
    Forfatter

M Achtman

  • Tilknyttet:
    Forfatter

Einar Ivanovitsj Rosenqvist

Bidragsyterens navn vises på dette resultatet som Einar Rosenqvist
  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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