Cristin-resultat-ID: 344782
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
2000

A Streptococcus pneumoniae heat shock protein 70 does not induce human antibody responses during infection

Bidragsytere:
  • Jan Isak Kolberg
  • Ernst Arne Høiby
  • Audun Aase
  • K Sletten
  • Gunnhild Målfrid Rødal
  • Terje Einar Michaelsen
  • mfl.

Tidsskrift

FEMS Immunology and Medical Microbiology
ISSN 0928-8244
NVI-nivå 0

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2000
Volum: 29
Sider: 289 - 294

Beskrivelse Beskrivelse

Tittel

A Streptococcus pneumoniae heat shock protein 70 does not induce human antibody responses during infection

Sammendrag

Mouse monoclonal antibodies (mAbs) were developed against Streptococcus pneumoniae in search for potential common pneumococcal proteins as vaccine antigens. mAb 230,B-9 (IgG1) reacted by immunoblotting with a 70-kDa protein which was isolated by immunoaffinity chromatography and subsequent preparative electrophoresis. N-terminal amino acid sequencing showed homology to that of heat shock protein 70 (hsp70). The hsp70 epitope reactive with mAb 230,B-9 was found in all the pneumococci examined as well as in other streptococci and enterococci. The epitope was not expressed in several other examined Gram-positive or -negative bacteria. Pneumococcal hsp70 has by other investigators been proposed to be a vaccine candidate. Binding experiments using flow cytometry showed that the epitope was not surface-exposed on live exponential phase grown S. pneumoniae. Human patient sera did not react with affinity-purified pneumococcal hsp70. Therefore the pneumococcal hsp70 does not seem to be of special interest in a vaccine formulation. The human sera contained antibodies to high molecular proteins co-purified with hsp70. Some of these proteins could be the pneumococcal surface protein A

Bidragsytere

Jan Isak Kolberg

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Ernst Arne Høiby

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

K Sletten

  • Tilknyttet:
    Forfatter

Gunnhild Målfrid Rødal

  • Tilknyttet:
    Forfatter
    ved Avdeling for bakteriologi ved Folkehelseinstituttet
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