Cristin-resultat-ID: 346124
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
2004

Selection and characterization of cyclic peptides that bind to a monoclonal antibody against meningococcal L3,7,9 lipopolysaccharides

Bidragsytere:
  • Vigdis Lauvrak
  • G Berntzen
  • Ulla Heggelund
  • Tove Karin Herstad
  • Randi Helene Sandin
  • Rolf Dalseg
  • mfl.

Tidsskrift

Scandinavian Journal of Immunology
ISSN 0300-9475
e-ISSN 1365-3083
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2004
Volum: 59
Sider: 373 - 384

Beskrivelse Beskrivelse

Tittel

Selection and characterization of cyclic peptides that bind to a monoclonal antibody against meningococcal L3,7,9 lipopolysaccharides

Sammendrag

There is still no general vaccine for prevention of disease caused by group-B meningococcal strains. Meningococcal lipopolysaccharides (LPSs) have received attention as potential vaccine candidates, but concerns regarding their safety have been raised. Peptide mimics of LPS epitopes may represent safe alternatives to immunization with LPS. The monoclonal antibody (MoAb) 9-2-L3,7,9 specific for Neisseria meningitidis LPS immunotype L3,7,9 is bactericidal and does not cross-react with human tissue. To explore the possibility of isolating peptide mimics of the epitope recognized by MoAb 9-2-L3,7,9, we have constructed two phage display libraries of six and nine random amino acids flanked by cysteines. Furthermore, we developed a system for the easy exchange of peptide-encoding sequences from the phage-display system to a hepatitis B core (HBc) expression system. Cyclic peptides that specifically bound MoAb 9-2-L3,7,9 at a site overlapping with the LPS-binding site were selected from both libraries. Three out of four tested peptides which reacted with MoAb 9-2-L3,7,9 were successfully presented as fusions to the immunodominant loop of HBc particles expressed in Escherichia coli. However, both peptide conjugates to keyhole limpet haemocyanin and HBc particle fusions failed to give an anti-LPS response in mice

Bidragsytere

Vigdis Lauvrak

  • Tilknyttet:
    Forfatter

Gøril Berntzen

Bidragsyterens navn vises på dette resultatet som G Berntzen
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (tidl. IMBV) ved Universitetet i Oslo

Ulla Heggelund

  • Tilknyttet:
    Forfatter

Tove Karin Herstad

  • Tilknyttet:
    Forfatter
    ved Avdeling for metodeutvikling og analyse ved Folkehelseinstituttet

Randi Helene Sandin

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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