Cristin-resultat-ID: 350557
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
1993

Human IgG3 can adopt disulfide bond pattern characteristic for IgG1 without resembling it in complement mediated cell lysis

Bidragsytere:
  • OH Brekke
  • B Bremnes
  • Audun Aase
  • Randi Helene Sandin
  • Terje Einar Michaelsen og
  • I Sandlie

Tidsskrift

Molecular Immunology
ISSN 0161-5890
e-ISSN 1872-9142
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 1993
Volum: 30
Sider: 1419 - 1425

Beskrivelse Beskrivelse

Tittel

Human IgG3 can adopt disulfide bond pattern characteristic for IgG1 without resembling it in complement mediated cell lysis

Sammendrag

In this paper we describe the construction of mouse-human IgG3 mutant antibodies resembling IgG1 in their disulfide bond pattern between the heavy and light chain (H-L) and between the two heavy chains (H-H). The effector functions of these mutant antibodies were compared to normal IgG3 and IgG1. Changing only the disulfide bond pattern between the heavy and light chains did not alter the ability to induce complement mediated cell lysis (CML), regardless of the amount of corresponding antigen that had been introduced to the surface of the target cells. However, alteration of the disulfide bond pattern between the two heavy chains had a large effect on CML due to shortening of the hinge from 62 to 15 amino acids. No difference between the mutants and normal antibodies in antibody-dependent cell-mediated cytotoxicity (ADCC) was observed. This suggests that IgG3 can adopt the H-L disulfide bond pattern of IgG1 without obtaining the CML activity characteristic for IgG1

Bidragsytere

Ole Henrik Brekke

Bidragsyterens navn vises på dette resultatet som OH Brekke
  • Tilknyttet:
    Forfatter

B Bremnes

  • Tilknyttet:
    Forfatter

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Randi Helene Sandin

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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