Cristin-resultat-ID: 351516
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
1993

Human IgG3 is decreased and IgG1, IgG2 and IgG4 are unchanged in molecular sizes of human IgG3 by mild reduction and reoxidation without a major change in effector functions

Bidragsytere:
  • Terje Einar Michaelsen
  • Lisbeth Meyer Næss og
  • Audun Aase

Tidsskrift

Molecular Immunology
ISSN 0161-5890
e-ISSN 1872-9142
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 1993
Volum: 30
Sider: 35 - 45

Beskrivelse Beskrivelse

Tittel

Human IgG3 is decreased and IgG1, IgG2 and IgG4 are unchanged in molecular sizes of human IgG3 by mild reduction and reoxidation without a major change in effector functions

Sammendrag

Purified proteins of the four human IgG subclasses were reduced under neutral conditions to break the interchain S-S bonds, followed by dialysis to allow reformation of S-S bonds (pr/o treatment). The IgG1, IgG2 and IgG4 proteins apparently reformed native molecules by pr/o treatment, while IgG3 formed molecules with significantly smaller size, as measured by HPLC gel filtration, compared to the autologous native proteins. The degree of shrinking of the pr/o IgG3 molecules varied and was most pronounced at low protein concn. In addition, the temp and the concn of reducing agent during the pr/o treatment had some influence on the molecular size. The effect is probably due to a conformational change of the 62 amino acid long hinge of IgG3. The effector activity of pr/o IgG2 and pr/o IgG3 was studied by employing chimeric, mouse V and human C regions, monoclonal antibodies with the same NIP-binding properties. Thus, the interaction between IgG and the complement system was unchanged both for pr/o IgG2 and pr/o IgG3, while the Fc-receptor-mediated antibody-dependent cellular cytotoxicity (ADCC) was depressed to the same degree for both pr/o IgG2 and pr/o IgG3. Conclusively, the alteration of the conformation of the IgG3 molecule by pr/o treatment had no major influence on its effector functions

Bidragsytere

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Lisbeth Meyer Næss

  • Tilknyttet:
    Forfatter
    ved Avdeling for vaksineforebyggbare sykdommer ved Folkehelseinstituttet

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet
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