Cristin-resultat-ID: 351517
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
1993

The extended hinge region of IgG3 is not required for high phagocytic capacity, but the heavy chains must be disulphide bonded

Bidragsytere:
  • Audun Aase
  • I Sandlie
  • L Norderhaug
  • OH Brekke og
  • Terje Einar Michaelsen

Tidsskrift

European Journal of Immunology
ISSN 0014-2980
e-ISSN 1521-4141
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 1993
Volum: 23
Sider: 1546 - 1551

Beskrivelse Beskrivelse

Tittel

The extended hinge region of IgG3 is not required for high phagocytic capacity, but the heavy chains must be disulphide bonded

Sammendrag

Fc gamma receptor (Fc gamma R) phagocytosis and respiratory burst were induced by chimeric mouse-human anti-(4-hydroxy-5-iodo-3-nitrophenyl) acetyl IgG3 antibodies with mutations in hinge and/or in CH1 region. IgG3 mutants with different hinge length ranging from 47 to 0 amino acids, an IgG3 molecule with an artificial hinge of just one cysteine residue (HM-1), and two hybrid IgG3 molecules with IgG4 hinge or IgG4 CH1-hinge were tested. Using the monocytic cell line U937 as effector cells, the mutated IgG3 molecules were very similar, revealing high activity, while the IgG3/IgG4 hybrids revealed a slightly reduced activity. However, the hingeless (0-h) mutant was negative, except after interferon-gamma stimulation when it became slightly positive. Interestingly, HM-1 was as active as the IgG3 mutants. With polymorphonuclear leucocytes (PMN) as effector cells we obtained some day-to-day variations, but all the IgG3 mutants were highly active, with the two shortest hinge mutants somewhat less active. The IgG3/IgG4 hybrid molecules revealed an intermediate activity, while IgG4 wild-type and the 0-h mutant were negative. However, the HM-1 molecule revealed an activity similar to that of the IgG3 mutants. The phagocytic activity of U937 was inhibited by monomeric IgG, indicating the importance of Fc gamma RI. In contrast, with PMN both blockage of Fc gamma RII and cleavage of Fc gamma RIII were required to significantly reduce the phagocytosis and respiratory burst, thus showing that both receptors contribute to the effect. These results demonstrate that the extended IgG3 hinge region is not necessary for a high phagocytic activity and that the major structural importance of the hinge is to connect the two heavy chains in this region

Bidragsytere

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Inger Sandlie

Bidragsyterens navn vises på dette resultatet som I Sandlie
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (tidl. IMBV) ved Universitetet i Oslo

L Norderhaug

  • Tilknyttet:
    Forfatter

Ole Henrik Brekke

Bidragsyterens navn vises på dette resultatet som OH Brekke
  • Tilknyttet:
    Forfatter

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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