Cristin-resultat-ID: 363610
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
2006

Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface

Bidragsytere:
  • Jan Isak Kolberg
  • Audun Aase
  • N Bergman
  • Tove Karin Herstad
  • Gunnhild Målfrid Rødal
  • R Frank
  • mfl.

Tidsskrift

Microbiology
ISSN 1350-0872
e-ISSN 1465-2080
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2006
Volum: 152
Hefte: 5
Sider: 1307 - 1317

Importkilder

Isi-ID: 000237618000008

Beskrivelse Beskrivelse

Tittel

Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface

Sammendrag

Enolase represents one of the anchorless surface proteins of Streptococcus pneumoniae and has previously been identified as a plasminogen-binding protein, endowing this pathogen with host proteolytic activity. In this study the mAb 245,C-6 (IgG1) was produced in a BALB/c mouse after immunizing with a protein fraction from S. pneumoniae. The mAb reacted with recombinant pneumococcal enolase both under non-denaturing and denaturing conditions. The epitope for the mAb was mapped to residues (55)DKSRYGGLG(63) of pneumococcal enolase using a peptide array. By applying the previously reported structure of enolase, this epitope was localized in a surface-exposed loop in each of the monomers of the octameric enolase. Previous immunoelectron microscopic studies, using polyclonal rabbit antibodies against enolase, depicted enolase on the cell surface but did not quantify the amount of surface-exposed enolase on viable pneumococci. Here, flow cytometry revealed no binding of mAb 245,C-6 to viable pneumococci, including TIGR4 and its non-encapsulated isogenic mutant, and only a minor increase of fluorescence intensity was measured when the polyclonal anti-enolase antibodies were used. In contrast, control antibodies recognizing the choline-binding proteins (CBPs) PspA and PspC showed high reactivities. The non-encapsulated TIGR4 did not show increased levels of antibody binding for mAb 245,C-6 or polyclonal anti-enolase antibodies, but revealed increased binding of polyclonal antibodies reacting with PspA or PspC. These results suggest that, compared to other surface-exposed proteins such as CBPs, the amount of enolase under the selected conditions is low. Flow cytometry, however, with FITC-labelled plasminogen demonstrated that the amount of surface-exposed enolase is important for plasminogen binding and, therefore, is also important for pneumococcal pathogenesis.

Bidragsytere

Jan Isak Kolberg

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Patrick Bergman

Bidragsyterens navn vises på dette resultatet som N Bergman
  • Tilknyttet:
    Forfatter

Tove Karin Herstad

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Gunnhild Målfrid Rødal

  • Tilknyttet:
    Forfatter
    ved Avdeling for bakteriologi ved Folkehelseinstituttet
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