Cristin-resultat-ID: 365352
Sist endret: 25. oktober 2016 14:34
NVI-rapporteringsår: 2008
Resultat
Vitenskapelig artikkel
2008

Differential binding of cytokines to environmentally relevant particles: A possible source for misinterpretation of in vitro results?

Bidragsytere:
  • Anette Kocbach
  • Annike Irene Totlandsdal
  • Marit Låg
  • Magne Refsnes og
  • Per E Schwarze

Tidsskrift

Toxicology Letters
ISSN 0378-4274
e-ISSN 1879-3169
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2008
Volum: 176
Hefte: 2
Sider: 131 - 137

Importkilder

Isi-ID: 000253005900004

Beskrivelse Beskrivelse

Tittel

Differential binding of cytokines to environmentally relevant particles: A possible source for misinterpretation of in vitro results?

Sammendrag

Inflammation is considered as a key event in adverse health effects associated with exposure to ambient particulate matter. The inflammatory potential of particles is often compared using in vitro cell systems, where the particle-induced release of pro-inflammatory cytokines is measured. A major concern in these assays is the potential of particles to bind cytokines, which may lead to an underestimation of the inflammatory potential. We therefore investigated the cytokine binding to a selection of particle samples, including particles collected from outdoor sources (wood combustion, traffic) and particles commonly used to model environmental sources (ultrafine carbon black, diesel, quartz), for a range of pro-inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-8). Furthermore, the influence of serum proteins and particle- and cytokine concentrations on the cytokine binding was studied. Cytokines primarily bound to carbonaceous particles (up to 85%), not to mineral particles. Furthermore, depending on the type of cytokine, the cytokine binding could be reduced partly or completely by adding serum proteins to the cell growth medium or particle suspensions. Based on these observations we recommend either to adjust culturing and exposure conditions to prevent cytokine binding, or to adjust the measured cytokine release by application of correction factors obtained from cytokine binding experiments.

Bidragsytere

Anette Kocbach Bølling

Bidragsyterens navn vises på dette resultatet som Anette Kocbach
  • Tilknyttet:
    Forfatter
    ved Avdeling for luft og støy ved Folkehelseinstituttet

Annike Irene Totlandsdal

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Marit Låg

  • Tilknyttet:
    Forfatter
    ved Avdeling for luft og støy ved Folkehelseinstituttet

Magne Arnold Refsnes

Bidragsyterens navn vises på dette resultatet som Magne Refsnes
  • Tilknyttet:
    Forfatter
    ved Avdeling for luft og støy ved Folkehelseinstituttet

Per Everhard Schwarze

Bidragsyterens navn vises på dette resultatet som Per E Schwarze
  • Tilknyttet:
    Forfatter
    ved Avdeling for luft og støy ved Folkehelseinstituttet
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