Cristin-resultat-ID: 369766
Sist endret: 16. juni 2008, 14:20
NVI-rapporteringsår: 2008
Resultat
Vitenskapelig artikkel
2008

Antigen analysis of Mycobacterium tuberculosis H37Rv culture filtrate proteins

Bidragsytere:
  • Hiwa Målen
  • Tina Søfteland og
  • Harald G Wiker

Tidsskrift

Scandinavian Journal of Immunology
ISSN 0300-9475
e-ISSN 1365-3083
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2008
Volum: 67
Hefte: 3
Sider: 245 - 252

Importkilder

Isi-ID: 000252901600005

Klassifisering

Vitenskapsdisipliner

Medisinsk mikrobiologi

Beskrivelse Beskrivelse

Tittel

Antigen analysis of Mycobacterium tuberculosis H37Rv culture filtrate proteins

Sammendrag

Culture filtrates of Mycobacterium tuberculosis H37Rv highly enriched with secreted proteins were used to identify antigens recognized by a serum pool from tuberculosis patients. Two different approaches were used to separate the culture filtrate protein mixture: 1) Proteins were fractionated according to their hydrophobicity using a HPLC-C18 chromatography column followed by separation based on their molecular mass by SDS-PAGE and subsequent immuno-blotting, or 2) Proteins were separated by two-dimensional gel electrophoresis, based on their isoelectric point and their molecular mass. Twenty serologically reactive proteins were ultimately identified by both methods, including 4 novel antigens. Further, to estimate the immunogenicity of the identified culture filtrate proteins the relative antibody quantities were measured using Image Master soft-ware. Our results show that the antibodies against proteins belonging to the antigen 85 complex were the most abundant in the serum of patients with active tuberculosis. The most immunogenic proteins in terms of high antibody-to-protein-ratio were Rv3881c and three lipoproteins Rv0934 (the 38 kDa antigen), Rv0932c (pstS2), and Rv3006 (LppZ). Rv3881c is located in the region of difference 1 (RD1) which is deleted from Mycobacterium bovis BCG, and is therefore a particularly promising candidate for development of serodiagnostic assays to detect active tuberculosis. The proteins from the M. tuberculosis H37Rv culture filtrate are strong candidates to be evaluated for improvement of the serodiagnostic tests of tuberculosis.

Bidragsytere

Hiwa Målen

  • Tilknyttet:
    Forfatter
    ved Gades institutt ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Helse Bergen HF - Haukeland universitetssykehus

Tina Søfteland

  • Tilknyttet:
    Forfatter
    ved Gades institutt ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Helse Bergen HF - Haukeland universitetssykehus

Harald Gotten Wiker

Bidragsyterens navn vises på dette resultatet som Harald G Wiker
  • Tilknyttet:
    Forfatter
    ved Gades institutt ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Helse Bergen HF - Haukeland universitetssykehus
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