Cristin-resultat-ID: 371842
Sist endret: 25. oktober 2016 14:34
Resultat
Vitenskapelig artikkel
2006

Comparison and correlation of Neisseria meningitidis serogroup B immunologic assay results and human antibody responses following three doses of the Norwegian meningococcal outer membrane vesicle vaccine MenBvac

Bidragsytere:
  • J, Findlow
  • S, Taylor
  • Audun Aase
  • R, Horton
  • Robert Heyderman
  • J, Southern
  • mfl.

Tidsskrift

Infection and Immunity
ISSN 0019-9567
e-ISSN 1098-5522
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2006
Volum: 74
Hefte: 8
Sider: 4557 - 4565

Importkilder

Isi-ID: 000239381400019

Beskrivelse Beskrivelse

Tittel

Comparison and correlation of Neisseria meningitidis serogroup B immunologic assay results and human antibody responses following three doses of the Norwegian meningococcal outer membrane vesicle vaccine MenBvac

Sammendrag

The prediction of efficacy of Neisseria meningitidis serogroup B (MenB) vaccines is currently hindered due to the lack of an appropriate correlate of protection. For outer membrane vesicle (OMV) vaccines, immunogenicity has primarily been determined by the serum bactericidal antibody (SBA) assay and OMV enzyme-linked immunosorbent assay (ELISA). However, the opsonophagocytic assay (OPA), surface labeling assay, whole blood assay (WBA), and salivary antibody ELISA have been developed although correlation with protection is presently undetermined. Therefore, the aim of the study was to investigate further the usefulness of, and relationships between, MenB immunologic assays. A phase II trial of the OMV vaccine, MenBvac, with proven efficacy was initiated to compare immunologic assays incorporating the vaccine and six heterologous strains. Correlations were achieved between the SBA assay, OMV ELISA, and OPA using human polymorphonuclear leukocytes and human complement but not between an OPA using HL60 phagocytic cells and baby rabbit complement. Correlations between the surface labeling assay, the SBA assay, and the OMV ELISA were promising, although target strain dependent. Correlations between the salivary antibody ELISA and other assays were poor. Correlations to the WBA were prevented since many samples had results greater than the range of the assay. The study confirmed the immunogenicity and benefit of a third dose of MenBvac against the homologous vaccine strain using a variety of immunologic assays. These results emphasize the need for standardized methodologies that would allow a more robust comparison of assays between laboratories and promote their further evaluation as correlates of protection against MenB disease.

Bidragsytere

J. Findlow

Bidragsyterens navn vises på dette resultatet som J, Findlow
  • Tilknyttet:
    Forfatter

S. Taylor

Bidragsyterens navn vises på dette resultatet som S, Taylor
  • Tilknyttet:
    Forfatter

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

R. Horton

Bidragsyterens navn vises på dette resultatet som R, Horton
  • Tilknyttet:
    Forfatter
    ved University of Bristol

Robert Heyderman

  • Tilknyttet:
    Forfatter
    ved University of Bristol
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