Cristin-resultat-ID: 378951
Sist endret: 25. oktober 2016 14:35
Resultat
Vitenskapelig artikkel
2005

Protection by natural human immunoglobulin m antibody to meningococcal serogroup B capsular polysaccharide in the infant rat protection assay is independent of complement-mediated bacterial lysis

Bidragsytere:
  • M Toropainen
  • L Saarinen
  • Elisabeth Wedege
  • Karin Bolstad
  • Terje Einar Michaelsen
  • Audun Aase
  • mfl.

Tidsskrift

Infection and Immunity
ISSN 0019-9567
e-ISSN 1098-5522
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2005
Volum: 73
Hefte: 8
Sider: 4694 - 4703

Beskrivelse Beskrivelse

Tittel

Protection by natural human immunoglobulin m antibody to meningococcal serogroup B capsular polysaccharide in the infant rat protection assay is independent of complement-mediated bacterial lysis

Sammendrag

Neisseria meningitidis, an important cause of bacterial meningitis and septicemia worldwide, is associated with high mortality and serious sequelae. Natural immunity against meningococcal disease develops with age, but the specificity and functional activity of natural antibodies associated with protection are poorly understood. We addressed this question by using a selected subset of prevaccination sera (n = 26) with convergent or discrepant serum bactericidal activity (SBA) and infant rat protective activity (IRPA) against the serogroup B meningococcal strain 44/76-SL (B:15:P1.7,16) from Icelandic teenagers. The sera were analyzed by opsonophagocytic activity (OPA) assay, immunoblotting, immunoglobulin G (IgG) quantitation against live meningococcal cells by flow cytometry, and enzyme immunosorbent assay (EIA). High levels of SBA and OPA were reflected in distinct IgG binding to major outer membrane proteins and/or lipopolysaccharide in immunoblots. However, we could not detect any specific antibody patterns on blots that could explain IRPA. Only IgM antibody to group B capsular polysaccharide (B-PS), measured by EIA, correlated positively (r = 0.76, P

Bidragsytere

M Toropainen

  • Tilknyttet:
    Forfatter

L Saarinen

  • Tilknyttet:
    Forfatter

Elisabeth Wedege

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Karin Bolstad

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Seksjon for farmasøytisk kjemi ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
1 - 5 av 7 | Neste | Siste »