Cristin-resultat-ID: 379146
Sist endret: 21. januar 2015 15:27
NVI-rapporteringsår: 2006
Resultat
Vitenskapelig artikkel
2006

Protection by natural human immunoglobulin m antibody to meningococcal serogroup B capsular polysaccharide in the infant rat protection assay is independent of complement-mediated bacterial lysis

Bidragsytere:
  • M Toropainen
  • L Saarinen
  • E Wedege
  • K Bolstad
  • Terje Einar Michaelsen
  • H Kayhty
  • mfl.

Tidsskrift

Infection and Immunity
ISSN 0019-9567
e-ISSN 1098-5522
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2006
Volum: 73
Sider: 4694 - 4703

Beskrivelse Beskrivelse

Tittel

Protection by natural human immunoglobulin m antibody to meningococcal serogroup B capsular polysaccharide in the infant rat protection assay is independent of complement-mediated bacterial lysis

Sammendrag

Neisseria meningitidis, an important cause of bacterial meningitis and septicemia worldwide, is associated with high mortality and serious sequelae. Natural immunity against meningococcal disease develops with age, but the specificity and functional activity of natural antibodies associated with protection are poorly understood. We addressed this question by using a selected subset of prevaccination sera (n = 26) with convergent or discrepant serum bactericidal activity (SBA) and infant rat protective activity (IRPA) against the serogroup B meningococcal strain 44/76-SL (B:15:P1.7,16) from Icelandic teenagers. The sera were analyzed by opsonophagocytic activity (OPA) assay, immunoblotting, immunoglobulin G (IgG) quantitation against live meningococcal cells by flow cytometry, and enzyme immunosorbent assay (EIA). High levels of SBA and OPA were reflected in distinct IgG binding to major outer membrane proteins and/or lipopolysaccharide in immunoblots. However, we could not detect any specific antibody patterns on blots that could explain IRPA. Only IgM antibody to group B capsular polysaccharide (B-PS), measured by EIA, correlated positively (r = 0.76, P

Bidragsytere

M Toropainen

  • Tilknyttet:
    Forfatter

L Saarinen

  • Tilknyttet:
    Forfatter

E Wedege

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

K Bolstad

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Terje Einar Michaelsen

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
  • Tilknyttet:
    Forfatter
    ved Seksjon for farmasøytisk kjemi ved Universitetet i Oslo
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