Cristin-resultat-ID: 419220
Sist endret: 21. januar 2015, 15:27
Resultat
Vitenskapelig artikkel
2002

Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish

Bidragsytere:
  • Anne Vatland Krøvel og
  • Lisbeth Charlotte Olsen

Tidsskrift

Mechanisms of Development
ISSN 0925-4773
e-ISSN 1872-6356
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2002
Volum: 116
Sider: 141 - 150

Beskrivelse Beskrivelse

Tittel

Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish

Sammendrag

In zebrafish, maternally produced vasa (vas) transcripts become targeted to the cleavage planes of early embryos and subsequently incorporated into the primordial germ cells (PGCs). Zygotic vas transcription occurs from the onset of gastrulation. Here, we report on the characterisation of the zebrafish vas locus. The gene consists of 27 exons, spans about 25kb, and contains two CpG-rich regions. We have used vas regulatory regions to establish transgenic zebrafish lines expressing enhanced green fluorescent protein (EGFP) in their PGCs. Maternally encoded vas::EGFP transcripts and VAS::EGFP protein segregate with the PGCs during embryogenesis. We find that the maternally deposited vas::EGFP transcripts are stable during embryogensis at least up to 50h of development. Vas::EGFP transcripts could not be detected in embryos that inherit the transgene from males, most likely due to the lack of one or more regulatory elements required for early zygotic expression. We show that vas::EGFP transcripts become enriched to the cleavage planes in early embryos, a finding that supported an RNA localisation signal localised within the vas region of these transcripts.

Bidragsytere

Anne Vatland Krøvel

  • Tilknyttet:
    Forfatter
    ved Sars internasjonale senter for marin molekylærbiologi ved Universitetet i Bergen

Lisbeth Charlotte Olsen

  • Tilknyttet:
    Forfatter
    ved Sars internasjonale senter for marin molekylærbiologi ved Universitetet i Bergen
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