Cristin-resultat-ID: 424963
Sist endret: 30. juni 2014, 09:13
Resultat
Vitenskapelig artikkel
2002

Antibacterial activity in four marine crustacean decapods

Bidragsytere:
  • Tor Haug
  • Anita K. Kjuul
  • Klara Stensvåg
  • Erling Sandsdalen og
  • Olaf B. Styrvold

Tidsskrift

Fish and Shellfish Immunology
ISSN 1050-4648
e-ISSN 1095-9947
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2002
Volum: 12
Sider: 371 - 385

Importkilder

Scopus-ID: 2-s2.0-0035984579

Beskrivelse Beskrivelse

Tittel

Antibacterial activity in four marine crustacean decapods

Sammendrag

A search for antibacterial activity in different body-parts of Pandalus borealis (northern shrimp), Pagurus bernhardus (hermit crab), Hyas araneus (spider crab) and Paralithodes camtschatica (king crab) was conducted. Dried samples were extracted with 60% (v/v) acetonitrile, containing 0.1% (v/v) trifluoroacetic acid, and further extracted and concentrated on C18 cartridges. Eluates from the solid phase extraction were tested for antibacterial, lysozyme and haemolytic activity. Antibacterial activity against Escherichia coli, Vibrio anguillarum, Corynebacterium glutamicum and Staphylococcus aureus was detected in extracts from several tissues in all species tested, but mainly in the haemolymph and haemocyte extracts. V. anguilarum and C. glutamicum were generally the most sensitive microorganisms. In P. borealis and P. bernhardus most of the active fractions were not affected by proteinase K treatment, while in H. araneus and P. camtschatica most fractions were sensitive to proteinase K treatment, indicating antibacterial factors of proteinaceous nature. In P. bernhardus the active fractions were generally heat labile, whereas in H. araneus the activities were resistant to heat. Differences between active extracts regarding hydrophobicity and sensitivity for heat and proteinase K treatment indicate that several compounds are responsible for the antibacterial activities detected. Lysozyme-like activity could be detected in some fractions and haemolytic activity against human red blood cells could be detected in haemolymph/haemocyte and exoskeleton extracts from all species tested.

Bidragsytere

Tor Haug

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap, fiskeri og økonomi ved UiT Norges arktiske universitet

Anita K. Kjuul

  • Tilknyttet:
    Forfatter

Klara Stensvåg

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap, fiskeri og økonomi ved UiT Norges arktiske universitet

Erling Sandsdalen

  • Tilknyttet:
    Forfatter
    ved NOFIMA

Olaf Bay Styrvold

Bidragsyterens navn vises på dette resultatet som Olaf B. Styrvold
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap, fiskeri og økonomi ved UiT Norges arktiske universitet
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