Cristin-resultat-ID: 430961
Sist endret: 13. februar 2018 16:52
Resultat
Vitenskapelig artikkel
2003

Comparison of functional immune responses in humans after intranasal and intramuscular immunisations with outer membrane vesicle vaccines against group B meningococcal disease

Bidragsytere:
  • Audun Aase
  • Lisbeth Meyer Næss
  • Randi H Sandin
  • Tove Karin Herstad
  • Fredrik Oftung
  • Johan Holst
  • mfl.

Tidsskrift

Vaccine
ISSN 0264-410X
e-ISSN 1873-2518
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2003
Volum: 21
Sider: 2051 - 2060

Beskrivelse Beskrivelse

Tittel

Comparison of functional immune responses in humans after intranasal and intramuscular immunisations with outer membrane vesicle vaccines against group B meningococcal disease

Sammendrag

A serogroup B meningococcal outer membrane vesicle (OMV) vaccine was delivered either intranasally or intramuscularly to 12 and 10 volunteers, respectively. The mucosal vaccine was given as four weekly doses followed by a fifth dose after 5 months; each dose consisted of OMVs equivalent to 250 g of protein. The intramuscular (i.m.) vaccine, consisting of the same OMVs but adsorbed to Al(OH)3, was administered as three doses each of 25 g of protein, with 6 weeks interval between first and second doses and the third dose after 10 months. Both groups of vaccinees demonstrated significant immune responses when measured as specific IgG antibodies against live meningococci, as serum bactericidal activity (SBA) and as opsonophagocytic activity. Two weeks after the last dose, the anti-meningococcal IgG concentrations were significantly higher in the i.m. group (median IgG concentration: 43.1 g/ml) than in the intranasal group (10.6 g/ml) (P=0.001). The corresponding opsonophagocytic activity was 7.0 and 3.0 (median log2 titre) (P=0.001), and the SBA was 5.0 and 2.0 (median log2 titre) (P=0.005), for the i.m. and intranasal groups, respectively. The last immunisation induced an enhanced immune response in the i.m. group, whereas the intranasal group showed no significant booster response. Accordingly, affinity maturation of anti-OMV-specific IgG antibodies was seen only after i.m. vaccination. The IgG1 subclass dominated the responses in both groups, whereas the significant IgG3 responses observed in the i.m. group were absent in the intranasal group. Although the intranasal OMV vaccination schedule used here induced functional immune responses relevant to protection, an improved vaccine formulation and/or a modified mucosal immunisation regimen may be needed to achieve a systemic effect comparable to that seen after three doses of intramuscular vaccination.

Bidragsytere

Audun Aase

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Lisbeth Meyer Næss

  • Tilknyttet:
    Forfatter
    ved Avdeling for vaksineforebyggbare sykdommer ved Folkehelseinstituttet

Randi Helene Sandin

Bidragsyterens navn vises på dette resultatet som Randi H Sandin
  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Tove Karin Herstad

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet

Fredrik Oftung

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsimmunologi ved Folkehelseinstituttet
  • Tilknyttet:
    Forfatter
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