Cristin-resultat-ID: 435039
Sist endret: 21. januar 2015 15:27
NVI-rapporteringsår: 2002
Resultat
Vitenskapelig artikkel
2002

Coeliac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues

Bidragsytere:
  • Eva Helene Arentz-Hansen
  • Stephen N. McAdam
  • Øyvind Molberg
  • Burkhard Fleckenstein
  • Knut E. A. Lundin
  • T.J. Jorgensen
  • mfl.

Tidsskrift

Gastroenterology
ISSN 0016-5085
e-ISSN 1528-0012
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2002
Volum: 123
Hefte: 3
Sider: 803 - 809

Importkilder

ForskDok-ID: 81109

Beskrivelse Beskrivelse

Tittel

Coeliac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues

Sammendrag

BACKGROUND & AIMS: Celiac disease is a gluten-induced enteropathy that shows a strong association with HLA-DQ2 and -DQ8. Gluten-specific T cells, invariably restricted by DQ2 or DQ8, can be isolated from celiac lesions. Such gut-derived T cells have a preference for recognition of gluten that has been specifically deamidated by tissue transglutaminase. Only a few gliadin T-cell epitopes have been identified by earlier work. The aim of this study was to perform a systematic characterization of DQ2-restricted T-cell epitopes in alpha- and gamma-gliadins. METHODS: Epitopes were identified by mass spectrometry analysis of peptide fragments of recombinant gliadins and by use of synthetic peptides. RESULTS: We identified several new gamma-gliadin epitopes and an additional alpha-gliadin epitope. Interestingly, these and the previously identified epitopes are not randomly scattered across the gliadins but cluster in regions of the proteins with high content of proline residues. CONCLUSIONS: Several DQ2-restricted T-cell epitopes exist in gliadin that are located in regions rich in proline. This likely reflects epitope selection at the levels of digestive and antigen-presenting cell processing, transglutaminase-mediated deamidation, and/or peptide binding to DQ2.

Bidragsytere

Eva Helene Arentz-Hansen

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Stephen N. McAdam

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Øyvind Molberg

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Burkhard Fleckenstein

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Knut E. A. Lundin

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo
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