Cristin-resultat-ID: 438763
Sist endret: 21. januar 2015 15:27
Resultat
Vitenskapelig artikkel
2001

Staining of celiac disease-relevant t cells by peptide-DQ2 multimers

Bidragsytere:
  • Hanne Quarsten
  • Stephen N. McAdam
  • T. Skeie Jensen
  • Eva Helene Arentz-Hansen
  • Øyvind Molberg
  • Knut E. A. Lundin
  • mfl.

Tidsskrift

Journal of Immunology
ISSN 0022-1767
e-ISSN 1550-6606
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2001
Volum: 167
Sider: 4861 - 4868

Importkilder

ForskDok-ID: 64326

Beskrivelse Beskrivelse

Tittel

Staining of celiac disease-relevant t cells by peptide-DQ2 multimers

Sammendrag

Gluten-specific T cells in the small intestinal mucosa are thought to play a central role in the pathogenesis of celiac disease (CD). The vast majority of these T cells recognize gluten peptides when presented by HLA-DQ2 (DQA1*05/DQB1*02), a molecule which immunogenetic studies have identified as conferring susceptibility to CD. We have previously identified and characterized three DQ2-restricted gluten epitopes that are recognized by intestinal T cells isolated from CD patients, two of which are immunodominant. Because almost all of the gluten epitopes are restricted by DQ2, and because we have detailed knowledge of several of these epitopes, we chose to develop peptide-DQ2 tetramers as a reagent to further investigate the role of these T cells in CD. In the present study, stable soluble DQ2 was produced such that it contained leucine zipper dimerization motif and a covalently coupled peptide. We have made four different peptide-DQ2 staining reagents, three containing the gluten epitopes and one containing a DQ2-binding self-peptide that provides a negative control for staining. We show in this study that peptide-DQ2 when adhered to plastic specifically stimulates T cell clones and that multimers comprising these molecules specifically stain peptide-specific T cell clones and lines. Interestingly, T cell activation caused severe reduction in staining intensities obtained with the multimers and an Ab to the TCR. The problem of TCR down-modulation must be taken into consideration when using class II multimers to stain T cells that may have been recently activated in vivo.

Bidragsytere

Hanne Quarsten

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Stephen N. McAdam

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Tone Skeie Jensen

Bidragsyterens navn vises på dette resultatet som T. Skeie Jensen
  • Tilknyttet:
    Forfatter

Eva Helene Arentz-Hansen

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Øyvind Molberg

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo
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