Cristin-resultat-ID: 439037
Sist endret: 7. januar 2002, 17:27
Resultat
Vitenskapelig artikkel
2000

High-resolution integrated map encompassing the breast cancer loss of heterozygosity region on human chromosome 16q22.1

Bidragsytere:
  • Eirik Frengen
  • Philippe Rocca-Serra
  • Sergey Shaposhnikov
  • L. Taine
  • Jim Thorsen
  • C. Bepoldin
  • mfl.

Tidsskrift

Genomics
ISSN 0888-7543
e-ISSN 1089-8646
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2000
Volum: 70
Hefte: 3
Sider: 273 - 285
Open Access

Importkilder

ForskDok-ID: 63413

Beskrivelse Beskrivelse

Tittel

High-resolution integrated map encompassing the breast cancer loss of heterozygosity region on human chromosome 16q22.1

Sammendrag

Loss of heterozygosity (LOH) on the long arm of human chromosome 16 is a common genetic alteration observed both in invasive ductal (IDC) and invasive lobular (ILC) breast carcinomas. We have generated a high-resolution integrated map encompassing the smallest region of LOH overlap within chromosome 16q22.1 (SRO2). Southern hybridization experiments using more than 140 probes resulted in the assembly of 152 bacterial large insert clones into a 2.8 Mb contig covering SRO2. The structure of the contig was verified by long range mapping using total human genomic DNA, and the contig orientation was determined by fluorescence in situ hybridization (FISH). A total of 68 transcripts have been identified in the map. One of the genes residing within SRO2 is the E-cadherin gene, CDH1, which has previously been shown to be mutated in lobular breast carcinomas, resulting in loss of E-cadherin expression. In most cases of ductal carcinoma, which is the major mammary cancer type, E-cadherin is normally expressed, suggesting other genes within 16q22.1 to be involved in the development of this tumor subtype. The high-resolution map presented in this study provides a valuable resource for identification of tumor suppressor genes expected to be involved in the etiology of breast carcinomas.

Bidragsytere

Eirik Frengen

  • Tilknyttet:
    Forfatter
    ved The Biotechnology Centre of Oslo (BiO): Common area ved Universitetet i Oslo

Philippe Rocca-Serra

  • Tilknyttet:
    Forfatter

Sergey Shaposhnikov

  • Tilknyttet:
    Forfatter
    ved The Biotechnology Centre of Oslo (BiO): Common area ved Universitetet i Oslo

L. Taine

  • Tilknyttet:
    Forfatter

Jim Thorsen

  • Tilknyttet:
    Forfatter
    ved The Biotechnology Centre of Oslo (BiO): Common area ved Universitetet i Oslo
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