Cristin-resultat-ID: 441288
Sist endret: 21. januar 2015 15:27
Resultat
Vitenskapelig artikkel
1999

Antigen-specific T-cell responses in humans after intranasal immunization with a meningococcal serogroup B outer membrane vesicle vaccine

Bidragsytere:
  • Fredrik Oftung
  • Lisbeth M. Naess
  • Lee M. Wetzler
  • Gro Ellen Korsvold
  • Audun Aase
  • Ernst Arne Høiby
  • mfl.

Tidsskrift

Infection and Immunity
ISSN 0019-9567
e-ISSN 1098-5522
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 1999
Volum: 67
Sider: 921 - 927

Importkilder

ForskDok-ID: 54296

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Tittel

Antigen-specific T-cell responses in humans after intranasal immunization with a meningococcal serogroup B outer membrane vesicle vaccine

Sammendrag

We have studied the ability of the Norwegian group B meningococcal outer membrane vesicle (OMV) vaccine, when administered intranasally without adjuvant, to induce T-cell responses in humans. A group of 12 vaccinees was immunized with four doses of OMVs (250 micrograms of protein/dose) at weekly intervals, and a single booster dose was given 5 months later. In vitro T-cell proliferation in response to the OMV vaccine, purified PorA (class 1) protein, PorB (class 3) protein, and one unrelated control antigen (Mycobacterium bovis BCG) was measured by [3H]thymidine incorporation into peripheral blood mononuclear cells obtained from the vaccinees before and after the immunizations. The nasal OMV immunizations induced antigen-specific T-cell responses in the majority of the vaccinees when tested against OMVs (7 of 12) and the PorA antigen (11 of 12). None of the vaccinees showed a vaccine-induced T-cell response to the PorB antigen after the initial four doses. Although some individuals responded to all the vaccine antigens after the booster dose, this response was not significant when the vaccinees were analyzed as a group. We have also demonstrated that the PorA antigen-specific T-cell responses correlated with anti-OMV immunoglobulin A (IgA) levels in nasal secretions, with anti-OMV IgG levels in serum, and with serum bactericidal activity. In conclusion, we have shown that it is possible to induce antigen-specific T-cell responses in humans by intranasal administration of a meningococcal OMV vaccine without adjuvant.

Bidragsytere

Fredrik Oftung

  • Tilknyttet:
    Forfatter

Lisbeth M. Naess

  • Tilknyttet:
    Forfatter

Lee M. Wetzler

  • Tilknyttet:
    Forfatter

Gro Ellen Korsvold

  • Tilknyttet:
    Forfatter

Audun Aase

  • Tilknyttet:
    Forfatter
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