Cristin-resultat-ID: 443132
Sist endret: 21. januar 2015 15:27
Resultat
Vitenskapelig artikkel
2000

Production of a panel of recombinant gliadins for the characterisation of T cell reactivity in coeliac disease

Bidragsytere:
  • Eva Helene Arentz-Hansen
  • Stephen N. McAdam
  • Øyvind Molberg
  • Christel Kristiansen og
  • Ludvig Magne Sollid

Tidsskrift

Gut
ISSN 0017-5749
e-ISSN 1468-3288
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2000
Volum: 46
Sider: 46 - 51

Importkilder

ForskDok-ID: 46734

Beskrivelse Beskrivelse

Tittel

Production of a panel of recombinant gliadins for the characterisation of T cell reactivity in coeliac disease

Sammendrag

BACKGROUND/AIMS: Coeliac disease is a chronic intestinal disorder most probably caused by an abnormal immune reaction to wheat gliadin. The identification of the HLA-DQ2 and HLA-DQ8 as the molecules responsible for the HLA association in coeliac disease strongly implicates a role for CD4 T cells in disease pathogenesis. Indeed, CD4 T cells specific for gliadin have been isolated from the small intestine of patients with coeliac disease. However, identification of T cell epitopes within gliadin has been hampered by the heterogeneous nature of the gliadin antigen. To aid the characterisation of gliadin T cell epitopes, multiple recombinant gliadins have been produced from a commercial Nordic wheat cultivar. METHODS: The alpha-gliadin and gamma-gliadin genes were amplified by polymerase chain reaction from cDNA and genomic DNA, cloned into a pET expression vector, and sequenced. Genes encoding mature gliadins were expressed in Escherichia coli and tested for recognition by T cells. RESULTS: In total, 16 alpha-gliadin genes with complete open reading frames were sequenced. These genes encoded 11 distinct gliadin proteins, only one of which was found in the Swiss-Prot database. Expression of these gliadin genes produced a panel of recombinant alpha-gliadin proteins of purity suitable for use as an antigen for T cell stimulation. CONCLUSION: This study provides an insight into the complexity of the gliadin antigen present in a wheat strain and has defined a panel of pure gliadin antigens that should prove invaluable for the future mapping of epitopes recognised by intestinal T cells in coeliac disease.

Bidragsytere

Eva Helene Arentz-Hansen

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Stephen N. McAdam

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Øyvind Molberg

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Christel Kristiansen

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo

Ludvig Magne Sollid

  • Tilknyttet:
    Forfatter
    ved Immunologisk institutt ved Universitetet i Oslo
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