Cristin-resultat-ID: 611638
Sist endret: 5. januar 2010 00:00
NVI-rapporteringsår: 2009
Resultat
Vitenskapelig artikkel
2009

Subcellular movement and expression of HSP27, alpha beta-crystallin, and HSP70 after two bouts of eccentric exercise in humans

Bidragsytere:
  • Gøran Paulsen
  • Fredrik Lauritzen
  • Monica Bayer
  • John Magne Kalhovde
  • Ingrid Ugelstad
  • Simen Gylterud Owe
  • mfl.

Tidsskrift

Journal of applied physiology
ISSN 8750-7587
e-ISSN 1522-1601
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2009
Volum: 107
Hefte: 2
Sider: 570 - 582

Importkilder

ForskDok-ID: r09019204

Klassifisering

Emneord

Stress • Proteiner

Beskrivelse Beskrivelse

Tittel

Subcellular movement and expression of HSP27, alpha beta-crystallin, and HSP70 after two bouts of eccentric exercise in humans

Sammendrag

Paulsen G, Lauritzen F, Bayer ML, Kalhovde JM, Ugelstad I, Owe SG, Hallen J, Bergersen LH, Raastad T. Subcellular movement and expression of HSP27, alpha beta-crystallin, and HSP70 after two bouts of eccentric exercise in humans. J Appl Physiol 107:570-582,2009. First published June 4, 2009; doi:10.1152/japplphysiol.00209.2009.-The aims of this study were to investigate the sarcomeric accumulation and expression of heat shock proteins (HSPs) after two bouts of maximal eccentric exercise. Twenty-four subjects performed two bouts of 70 maximal voluntary eccentric actions using the elbow flexors in one arm. The bouts were separated by 3 wk. The changes in concentric (60 degrees/s) and isometric (90 degrees) force-generating capacity were monitored for 9 days after each bout, and biopsies were taken 1 and 48 h and 4 and 7 days after bout 1 and 1 and 48 h after bout 2. The content of HSP27, alpha B-crystallin, HSP70, and desmin in the cytosolic and cytoskeleton/myofibrillar fractions of homogenized muscle samples was determined by immunoassays, and the cellular and subcellular localization of the HSPs in the myofibrillar structure was analyzed by conventional and confocal immunofluorescence microscopy and quantitative electron microscopy. The force-generating capacity was reduced by similar to 50% and did not recover completely during the 3 wk following bout 1. After bout 2, the subjects recovered within 4 days. The HSP levels increased in the cytosolic fraction after bout 1, especially HSP70 (similar to 300% 2-7 days after exercise). Increased levels of HSP27, alpha B-crystallin, and HSP70 were found in the cytoskeletal/myofibrillar fraction after both bouts, despite reduced damage after bout 2. At the ultrastructural level, HSP27 and alpha B-crystallin accumulated in Z-disks, in intermediate desmin-like structures (alpha B-crystallin), and in areas of myofibrillar disruption. In conclusion, HSP27 and alpha B-crystallin accumulated in myofibrillar structures, especially in the Z-disks and the intermediate structures (desmin). The function of the small HSPs is possibly to stabilize and protect the myofibrillar structures during and after unaccustomed eccentric exercise. The large amount of HSP27, alpha B-crystallin, and HSP70 in the cytoskeletal/myofibrillar fraction after a repeated bout of exercise suggests a protective role as part of the repeated-bout effect.

Bidragsytere

Gøran Paulsen

  • Tilknyttet:
    Forfatter
    ved Institutt for fysisk prestasjonsevne ved Norges idrettshøgskole
Inaktiv cristin-person

Fredrik Lauritzen

  • Tilknyttet:
    Forfatter
    ved Institutt for fysisk prestasjonsevne ved Norges idrettshøgskole

Monica Bayer

  • Tilknyttet:
    Forfatter
    ved Bispebjerg Hospital
  • Tilknyttet:
    Forfatter
  • Tilknyttet:
    Forfatter
    ved Institutt for fysisk prestasjonsevne ved Norges idrettshøgskole

John Magne Kalhovde

  • Tilknyttet:
    Forfatter
    ved Institutt for fysisk prestasjonsevne ved Norges idrettshøgskole

Ingrid Ugelstad

  • Tilknyttet:
    Forfatter
    ved Institutt for fysisk prestasjonsevne ved Norges idrettshøgskole
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