Cristin-resultat-ID: 617472
Sist endret: 2. juni 2017, 12:09
Resultat
Poster
2007

Association of Providencia alcalifaciens with diarrhea in dogs

Bidragsytere:
  • Trine Marie L'Abée-Lund
  • Aud Kari Fauske
  • Marit Næve Hirsch og
  • Simon P. Hardy

Presentasjon

Navn på arrangementet: 2007 Conference of Gastrointestinal Function
Sted: Chicago, Illinois, USA
Dato fra: 16. april 2007
Dato til: 18. april 2007

Om resultatet

Poster
Publiseringsår: 2007

Importkilder

ForskDok-ID: r08009628

Beskrivelse Beskrivelse

Tittel

Association of Providencia alcalifaciens with diarrhea in dogs

Sammendrag

During the past 20 years, Providencia alcalifaciens in the family Enterobacteriaceae has been suggested as a primary enteric pathogen in humans. The bacterium has been associated with traveler?s diarrhea and diarrhea in children from developing countries, but also a large outbreak of foodborne infection has been reported (Heynes and Hawkey 1989, Albert et al 1998, Murata et al 2001). Strains of P. alcalifaciens are shown to be invasive in cell studies, but other virulence mechanisms have not been detected (Janda et al 1998). In September 2005 a rectal swab of a dog with bloody diarrhea grew P. alcalifaciens as the predominant organism. Subsequently, this was also seen in additional 13 fecal specimens from dogs with severe diarrhea. Two of the dogs were from the same household, but no connections are known between the other dogs. Möhr et al (2002) report an incidence of primary bacterial enteritis possibly caused by P. alcalifaciens in three puppies, while Tribe and Rood (2002) conclude that this species should not be considered as a primary pathogen in dogs. The aim of this study was to investigate the 14 isolates of P. alcalifaciens from dogs with diarrhea. Pulsed field gel electrophoresis (PFGE) was performed on genomic DNA from all isolates after digestion with SmaI restriction enzyme. Representative isolates were tested for enterotoxin production and cell invasive ability in Vero cells. PFGE showed that ten of the isolates were closely related, nine of these were showed to be indistinguishable by visual examination of the band patterns, while one isolate differed by the lack of one band. No enterotoxin production was seen. The bacteria were able to invade Vero cells. The clonal appearance of the majority of these isolates indicates that P. alcalifaciens may be an enteric pathogen also in dogs.

Bidragsytere

Trine Marie LAbée-Lund Normann

Bidragsyterens navn vises på dette resultatet som Trine Marie L'Abée-Lund
  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Aud Kari Fauske

  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Marit Næve Hirsch

  • Tilknyttet:
    Forfatter
    ved Norges miljø- og biovitenskapelige universitet

Simon P. Hardy

  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet
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