Cristin-resultat-ID: 680363
Sist endret: 30. mai 2017, 11:31
Resultat
Poster
2009

Expression of genes involved in Atlantic halibut (Hippoglossus hippoglossus) metabolism, a fasting and refeeding study

Bidragsytere:
  • Ørjan Hagen
  • Jorge Fernandes
  • Christel Solberg og
  • Ian A. Johnston

Presentasjon

Navn på arrangementet: Genomics in Aquaculture 2009
Sted: Bodø, Norway
Dato fra: 5. juli 2009
Dato til: 7. juli 2009

Om resultatet

Poster
Publiseringsår: 2009

Importkilder

ForskDok-ID: r09020556

Beskrivelse Beskrivelse

Tittel

Expression of genes involved in Atlantic halibut (Hippoglossus hippoglossus) metabolism, a fasting and refeeding study

Sammendrag

The aim of this study was to establish a fasting-refeeding protocol to investigate the expression of growth-related genes during the transition between catabolic and anabolic states in Atlantic halibut (Hippoglossus hippoglossus L.). Juveniles of ~950g were maintained at ambient temperature (5-8¿C) until the 1st May, then fasted for two months and refed for two months at 7.7-8.0¿C under continuous light. Fast epaxial myotomal muscle was sampled at -64 days (d), -38d (fasting), 0d (start of refeeding), 3d, 7d, 14d, 30d and 60d (refeeding). Average body mass was unchanged over the fasting period but increased by 24.4% following 60d refeeding. qPCR was used to analyse the stability of expression of five potential reference genes (Eef2, Fau, 18S rRNA, Actb and Tubb2) with GeNorm and Normfinder. Expression of the growth-related genes, cathepsin B (ctsb), cathepsin D (ctsd), insulin-like growth factor-I and II (IGF-I & II) and insulin-like growth factor-I receptor 1a (IGF-IRa) was normalised using the geometric average of the two most stable house keeping genes, Fau and 18S rRNA. IGF-I mRNA showed a transient 2.6-fold increase in abundance with refeeding at 7d whilst transcripts for IGF-II and IGF-IRa were elevated during fasting and decreased 3.8-fold and 3-fold between the 0d and 3d samples respectively. Ctsb expression increased between -64d and 0d and then decreased ~10-fold by 14d refeeding. In contrast, ctsd was relatively unaffected by the fasting-refeeding cycle, showing a modest (~35%) transient decrease in expression between the 0d and 30d refeeding samples. It was concluded that the experimental protocol adopted and housekeeping genes identified were suitable for investigating the catabolic-anabolic transition in halibut skeletal muscle

Bidragsytere

Ørjan Hagen

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

Bidragsyterens navn vises på dette resultatet som Jorge Fernandes
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Christel Solberg

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Ian A. Johnston

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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