Cristin-resultat-ID: 728473
Sist endret: 22. oktober 2008 00:00
NVI-rapporteringsår: 2008
Resultat
Vitenskapelig artikkel
2008

Simulating dark expressions and interactions of frq and wc-1 in the Neurospora circadian clock

Bidragsytere:
  • Christian I. Hong
  • Ingunn Jolma
  • Jennifer J. Loros
  • Jay C. Dunlap og
  • Peter Ruoff

Tidsskrift

Biophysical Journal
ISSN 0006-3495
e-ISSN 1542-0086
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2008
Volum: 94
Hefte: 4
Sider: 1221 - 1232

Importkilder

ForskDok-ID: r08018105

Beskrivelse Beskrivelse

Tittel

Simulating dark expressions and interactions of frq and wc-1 in the Neurospora circadian clock

Sammendrag

Circadian rhythms are considered to play an essential part in the adaptation of organisms to their environments. The occurrence of circadian oscillations appears to be based on the presence of transcriptional-translational negative feedback loops. In Neurospora crassa, the protein FREQUENCY (FRQ) is part of such a negative feedback loop apparently by a direct interaction with its transcription factor WHITE COLLAR-1 (WC-1). Based on the observation that nuclear FRQ levels are significantly lower than nuclear WC-1 levels, it was suggested that FRQ would act more like a catalyst in inhibiting WC-1 rather than binding to WC-1 and making an inactive FRQ: WC-1 complex. Intrigued by this hypothesis, we constructed a model for the Neurospora circadian clock, which includes expression of the frq and the wc-1 genes and their possible interactions. The model suggests that even small amounts of nuclear FRQ-protein are capable of inhibiting frq transcription in a rhythmic manner by binding to WC-1 and promoting its degradation. Our model predicts the importance of a FRQ dependent degradation of WC-1 in closing the negative feedback loop. The model shows good agreement with experimental levels in nuclear and cytosolic FRQ and WC-1, their phase relationships, and several clock mutant phenotypes.

Bidragsytere

Christian I. Hong

  • Tilknyttet:
    Forfatter
  • Tilknyttet:
    Forfatter
    ved Institutt for matematikk og fysikk ved Universitetet i Stavanger

Ingunn Westvik Jolma

Bidragsyterens navn vises på dette resultatet som Ingunn Jolma
  • Tilknyttet:
    Forfatter
    ved Institutt for matematikk og fysikk ved Universitetet i Stavanger

Jennifer J. Loros

  • Tilknyttet:
    Forfatter

Jay C. Dunlap

  • Tilknyttet:
    Forfatter

Heinz Peter Ruoff

Bidragsyterens navn vises på dette resultatet som Peter Ruoff
  • Tilknyttet:
    Forfatter
    ved Institutt for matematikk og fysikk ved Universitetet i Stavanger
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