Cristin-resultat-ID: 771830
Sist endret: 2. juni 2017, 13:03
NVI-rapporteringsår: 2011
Resultat
Vitenskapelig artikkel
2011

Multilocus sequence analyses reveal several unnamed Mesorhizobium genospecies nodulating Acacia species and Sesbania sesban trees in Southern regions of Ethiopia

Bidragsytere:
  • Tulu Degefu Abdi
  • Endalkachew Woldemeskel og
  • Åsa Frostegård

Tidsskrift

Systematic and Applied Microbiology
ISSN 0723-2020
e-ISSN 1618-0984
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2011
Volum: 34
Hefte: 3
Sider: 216 - 226

Importkilder

Isi-ID: 000291710700007
ForskDok-ID: r11007631
Scopus-ID: 2-s2.0-79954576283

Beskrivelse Beskrivelse

Tittel

Multilocus sequence analyses reveal several unnamed Mesorhizobium genospecies nodulating Acacia species and Sesbania sesban trees in Southern regions of Ethiopia

Sammendrag

Leguminous trees play an important role in agroforestry in Ethiopia, but studies of their rhizobial symbionts are scarce. In earlier studies, we surveyed natural nodulation of native leguminous trees growing in different agro-ecological zones in Southern Ethiopia, isolated 400 rhizobia, and characterized them based on different phenotypic and genotypic methods. In the present study we characterized 18 strains belonging to the genus Mesorhizobium, isolated from nodules of Acacia abyssinica, A. senegal, A. tortilis and Sesbania sesban. Phylogenetic analysis of nearly full-length 16S rRNA gene grouped the test strains into three distinct clades separated from all currently recognized Mesorhizobium species. Three divergent strains formed separate branches while the other 15 strains formed three distinct groups, genospecies I–III. Grouping of the isolates under study based on the house-keeping genes recA, gyrB, rpoB and gltA were consistent and in agreement with that of 16S rRNA. Similarly phylogenetic relationships based on the symbiosis-related genes nodC, nodA and nifH were generally similar to those shown by the core genes, suggesting that these Acacia and Sesbania symbionts have a long history of separate evolution within Mesorhizobium. Cross inoculation experiments demonstrated a large variation in the ability of the test strains to elicit effective nodules. The Sesbania isolates, occupying a distinct clade in the nodC phylogenetic tree, formed effective nodules only with this host legume. The study strongly suggests that this collection of Mesorhizobium strains comprises several new species, and also indicates the role of the symbiotic genes in determining the host range of these bacteria.

Bidragsytere

Tulu Degefu

Bidragsyterens navn vises på dette resultatet som Tulu Degefu Abdi
  • Tilknyttet:
    Forfatter
    ved Kjemi, bioteknologi og matvitenskap ved Norges miljø- og biovitenskapelige universitet

Endalkachew Woldemeskel

  • Tilknyttet:
    Forfatter
    ved Kjemi, bioteknologi og matvitenskap ved Norges miljø- og biovitenskapelige universitet
  • Tilknyttet:
    Forfatter
    ved Hawassa University

Åsa Helena Frostegård

Bidragsyterens navn vises på dette resultatet som Åsa Frostegård
  • Tilknyttet:
    Forfatter
    ved Kjemi, bioteknologi og matvitenskap ved Norges miljø- og biovitenskapelige universitet
1 - 3 av 3