Cristin-resultat-ID: 917467
Sist endret: 27. mars 2012 10:55
Resultat
Vitenskapelig foredrag
2011

Diversity and distribution of nano- and picoeukaryotes in the Southern Ocean as revealed by 454-pyrosequencing, clone libraries and microscopy

Bidragsytere:
  • Bente Edvardsen
  • Lars Nersveen
  • Tonie Leonora Torgrimsby
  • Anette Engesmo
  • Elianne Sirnæs Egge og
  • Wenche Eikrem

Presentasjon

Navn på arrangementet: International Conference, Phycological Society of America
Sted: Seattle
Dato fra: 12. juli 2011
Dato til: 16. juli 2011

Arrangør:

Arrangørnavn: Phycological Society of America

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2011

Beskrivelse Beskrivelse

Tittel

Diversity and distribution of nano- and picoeukaryotes in the Southern Ocean as revealed by 454-pyrosequencing, clone libraries and microscopy

Sammendrag

In this study we explored the diversity and distribution of planktonic nano- and picoeukaryotes in Antarctic waters by combining 454-pyrosequencing, clone libraries and electron microscopy. Water samples were collected and physico-chemical parameters measured during summer 2008 with the Norwegian R/V G.O. Sars in the Atlantic sector of the Southern Ocean, at stations along a transect (15°E) between 45-67o S. Ribosomal DNA of plankton in the size range 0.8-35µm was amplified with universal eukaryotic and haptophyte specific primers followed by pyro- and Sanger sequencing. Most rDNA sequences could not be assigned to a cultured and sequenced species (at ≥99,3% identity level) indicating that a large fraction of the plankton community remains to be genetically characterized. Electron microscopy revealed some of this diversity, but more cultured strains are needed as reference for a precise identification. The taxonomic groups represented with highest number of clones/reads were dinoflagellates, stramenopiles and haptophytes. The species composition was significantly different at the northernmost station compared to further south, suggesting that nano- and picoeukaryote species composition and distribution may serve as indicators of climate change in polar waters.

Bidragsytere

Aktiv cristin-person

Bente Edvardsen

  • Tilknyttet:
    Forfatter
    ved Marinbiologi ved Universitetet i Oslo

Lars Nersveen

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (tidl. BIO) ved Universitetet i Oslo

Tonie Leonora Torgrimsby

  • Tilknyttet:
    Forfatter

Anette Engesmo

  • Tilknyttet:
    Forfatter

Elianne Dunthorn Egge

Bidragsyterens navn vises på dette resultatet som Elianne Sirnæs Egge
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (tidl. BIO) ved Universitetet i Oslo
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