Cristin-resultat-ID: 917496
Sist endret: 27. mars 2012 11:53
Resultat
Poster
2011

QUANTIFICATION OF HAPTOPHYTE SPECIES BY PYROSEQUENCING: IS READ ABUNDANCE PROPORTIONAL TO SPECIES BIOVOLUME?

Bidragsytere:
  • Bente Edvardsen og
  • Elianne Sirnæs Egge

Presentasjon

Navn på arrangementet: Jacques Monod Conference- Integrative Genomic Ecology
Sted: Roscoff
Dato fra: 11. oktober 2011
Dato til: 15. oktober 2011

Arrangør:

Arrangørnavn: Jacques Monod Conference

Om resultatet

Poster
Publiseringsår: 2011

Beskrivelse Beskrivelse

Tittel

QUANTIFICATION OF HAPTOPHYTE SPECIES BY PYROSEQUENCING: IS READ ABUNDANCE PROPORTIONAL TO SPECIES BIOVOLUME?

Sammendrag

Amplicon-sequencing of ribosomal RNA genes has become a standard for assessing microbial diversity in environmental samples. Abundance is of interest in addition to presence/absence of species to assess their ecological importance. The proportion of DNA sequences/reads from a particular species may, however, not reflect the proportion of the species in a sample in terms of cell number. We investigated the relationship between cell number and biovolume in an artificial community of haptophyte species, and the number of DNA sequences/reads appearing in libraries produced by cloning and 454-pyrosequencing nucleic acids extracted from this community. One million cells from each of 11 haptophyte species were pooled, and DNA and RNA extracted, and the V4 region of the small subunit ribosomal gene was amplified using haptophyte specific primers. The amplicons were sequenced by 454-pyrosequencing or Sanger sequencing. Pyrosequencing with 17000 reads per sample recovered all species in the culture mix, whereas clone libraries with 50-80 sequences per sample only recovered

Bidragsytere

Aktiv cristin-person

Bente Edvardsen

  • Tilknyttet:
    Forfatter
    ved Marinbiologi ved Universitetet i Oslo

Elianne Dunthorn Egge

Bidragsyterens navn vises på dette resultatet som Elianne Sirnæs Egge
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (tidl. BIO) ved Universitetet i Oslo
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