Cristin-resultat-ID: 973192
Sist endret: 2. januar 2013, 12:17
NVI-rapporteringsår: 2012
Resultat
Vitenskapelig artikkel
2012

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Bidragsytere:
  • Saira Khan
  • Anne Tøndervik
  • Håvard Sletta
  • Geir Klinkenberg
  • Charlotte Emanuel
  • Edvar Onsøyen
  • mfl.

Tidsskrift

Antimicrobial Agents and Chemotherapy
ISSN 0066-4804
e-ISSN 1098-6596
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2012
Volum: 56
Hefte: 10
Sider: 5134 - 5141

Importkilder

Isi-ID: 000308807900019
Scopus-ID: 2-s2.0-84866342560

Beskrivelse Beskrivelse

Tittel

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Sammendrag

The uncontrolled, often inappropriate use of antibiotics has resulted in the increasing prevalence of antibiotic-resistant pathogens, with major cost implications for both United States and European health care systems. We describe the utilization of a low-molecular-weight oligosaccharide nanomedicine (OligoG), based on the biopolymer alginate, which is able to perturb multidrug-resistant (MDR) bacteria by modulating biofilm formation and persistence and reducing resistance to antibiotic treatment, as evident using conventional and robotic MIC screening and microscopic analyses of biofilm structure. OligoG increased (up to 512-fold) the efficacy of conventional antibiotics against important MDR pathogens, including Pseudomonas, Acinetobacter, and Burkholderia spp., appearing to be effective with several classes of antibiotic (i.e., macrolides, β-lactams, and tetracyclines). Using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), increasing concentrations (2%, 6%, and 10%) of alginate oligomer were shown to have a direct effect on the quality of the biofilms produced and on the health of the cells within that biofilm. Biofilm growth was visibly weakened in the presence of 10% OligoG, as seen by decreased biomass and increased intercellular spaces, with the bacterial cells themselves becoming distorted and uneven due to apparently damaged cell membranes. This report demonstrates the feasibility of reducing the tolerance of wound biofilms to antibiotics with the use of specific alginate preparations.

Bidragsytere

Saira Khan

  • Tilknyttet:
    Forfatter
    ved Cardiff University

Anne Tøndervik

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Håvard Sletta

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Geir Klinkenberg

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Charlotte Emanuel

  • Tilknyttet:
    Forfatter
    ved Cardiff University
1 - 5 av 11 | Neste | Siste »