Chromatic differences introduced by microscope optics

In this study we determined the chromatic difference introduced by the optics of two different microscopes: Olympus SZX10® and Nikon ECLIPSE MA200®, by carefully measuring the 24 different colours of the GretagMacbeth ColorChecker® with a spectroradiometer through the observation eyepiece of the microscopes and computing the chromatic and colour differences with the measured values of the patches without the microscope. The results obtained for the Olympus SZX10® microscope show a mean chromatic difference of 6.52, 4.45, 5.56, 3.52, 3.85, 4.22, and 4.48 units; and a mean colour difference of 7.56, 5.60, 9.55, 4.99, 4.98, 5.48, and 5.69 units for CIELAB, CMC, BFD, CIE94, CIEDE2000, DIN99d and DIN99b, respectively. On the other hand the results obtained for the Nikon ECLIPSE MA200® microscope show a mean chromatic difference of 10.34, 6.48, 8.30, 5.28, 6.15, 3.72, and 6.86 units; and a mean colour difference of 13.31, 9.45, 17.19, 9.22, 9.04, 7.87, and 10.24 units for CIELAB, CMC, BFD, CIE94, CIEDE2000, DIN99d and DIN99b, respectively.