Cristin-resultat-ID: 1129249
Sist endret: 31. mai 2017, 08:37
NVI-rapporteringsår: 2014
Resultat
Vitenskapelig artikkel
2014

Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Bidragsytere:
  • Kazue Nagasawa
  • Christopher Presslauer
  • Lech Kirtiklis
  • Igor Babiak og
  • Jorge Fernandes

Tidsskrift

Journal of Molecular Endocrinology
ISSN 0952-5041
e-ISSN 1479-6813
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2014
Volum: 52
Hefte: 3
Sider: 357 - 371
Open Access

Importkilder

Scopus-ID: 2-s2.0-84901427275

Beskrivelse Beskrivelse

Tittel

Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle

Sammendrag

The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod (Gadus morhua L.), a batch spawner, throughout annual reproductive cycle. Three nuclear ER partial cDNA sequences (esr1, esr2a and esr2b) were cloned and all esr transcripts were detected mainly in liver and gonads of either sex. In situ hybridization of esrs along with germ cell (vasa) and gonadal somatic cell markers (gsdf, 3β-hsd and amh for testicular, or gsdf for ovarian somatic cells) showed that in testis all three esrs were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells, whereas in ovary they were differentially expressed in both follicular cells and oocytes. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogues in testis and ovary. A significant increase in esr2a expression was identified in testis and esr2b in ovary, whereas esr1 transcripts were elevated in both testis and ovary at February and March prior to the spawning period. The localization and sexually dimorphic expression of esr genes in gonads suggest a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod.

Bidragsytere

Kazue Nagasawa

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Christopher Edward Presslauer

Bidragsyterens navn vises på dette resultatet som Christopher Presslauer
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Lech Kirtiklis

  • Tilknyttet:
    Forfatter
    ved Uniwersytet Warminsko-Mazurski w Olsztynie

Igor Szczepan Babiak

Bidragsyterens navn vises på dette resultatet som Igor Babiak
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

Bidragsyterens navn vises på dette resultatet som Jorge Fernandes
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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