Cristin-resultat-ID: 1142372
Sist endret: 28. november 2017, 10:56
NVI-rapporteringsår: 2014
Resultat
Vitenskapelig artikkel
2014

Reliable determination of training-induced alterations in muscle fiber composition in human skeletal muscle using quantitative polymerase chain reaction

Bidragsytere:
  • Stian Ellefsen
  • Olav Vikmoen
  • Erika Zacharoff
  • Irene Rauk
  • Gunnar Slettaløkken
  • Daniel Hammarström
  • mfl.

Tidsskrift

Scandinavian Journal of Medicine & Science in Sports
ISSN 0905-7188
e-ISSN 1600-0838
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2014
Volum: 24
Hefte: 4
Sider: e332 - e342

Importkilder

Scopus-ID: 2-s2.0-84928824335

Beskrivelse Beskrivelse

Tittel

Reliable determination of training-induced alterations in muscle fiber composition in human skeletal muscle using quantitative polymerase chain reaction

Sammendrag

Determination of muscle fiber composition in human skeletal muscle biopsies is often performed using immunohistochemistry, a method that tends to be both time consuming, technically challenging, and complicated by limited availability of tissue. Here, we introduce quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based Gene-family profiling (GeneFam) of myosin heavy chain (MyHC) mRNA expression as a high-throughput, sensitive, and reliable alternative. We show that GeneFam and immunohistochemistry result in similar disclosures of alterations in muscle fiber composition in biopsies from musculus vastus lateralis and musculus biceps brachii of previously untrained young women after 12 weeks of progressive strength training. The adaptations were evident as (a) consistent increases in MyHC2A abundance; (b) consistent decreases in MyHC2X abundance; and (c) consistently stable MyHC1 abundance, and were not found using traditional reference gene-based qRT-PCR analyses. Furthermore, muscle fiber composition found using each of the two approaches was correlated with each other (r = 0.50, 0.74, and 0.78 for MyHC1, A, and X, respectively), suggesting that GeneFam may be suitable for ranking of individual muscle phenotype, particularly for MyHC2 fibers. In summary, GeneFam of MyHC mRNA resulted in reliable assessment of alterations in muscle fiber composition in skeletal muscle of previously untrained women after 12 weeks of strength training.

Bidragsytere

Aktiv cristin-person

Stian Ellefsen

  • Tilknyttet:
    Forfatter
    ved Institutt for folkehelse- og idrettsvitenskap ved Høgskolen i Innlandet
Aktiv cristin-person

Olav Vikmoen

  • Tilknyttet:
    Forfatter
    ved Fakultet for helse- og sosialvitenskap ved Høgskolen i Innlandet

Erika Zacharoff

  • Tilknyttet:
    Forfatter
    ved Fakultet for lærerutdanning og pedagogikk ved Høgskolen i Innlandet

Irene Rauk

  • Tilknyttet:
    Forfatter
    ved Div Medisinsk service ved Sykehuset Innlandet HF

Gunnar Slettaløkken

  • Tilknyttet:
    Forfatter
    ved Institutt for folkehelse- og idrettsvitenskap ved Høgskolen i Innlandet
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