Sammendrag
Robust winter survival is necessary for efficient production of commercial strawberries. To identify proteins responsible for freezing tolerance in strawberry we have examined alterations in protein levels in strawberry varieties that differ in cold tolerance. Short and long term cold response was examined in mature plants by treating for 48 hours and 6 weeks at 4C. We made whole extracts from the crown tissue, as this is a major overwintering structure. Protein profiles were evaluated by using either 2-DE gel analysis followed by MALDI-TOF and LC-MS/MS analysis or a shotgun MS/MS approach. The 2-DE results suggest about 50 proteins out of 1000 are altered significantly in the cold tolerant variety. The shotgun results for the 48 h time point showed 44 significant changes of the 2017 proteins. We also identified proteins with distinct levels in the different varieties. Additional analysis includes functional categorization and principal component analysis. Transcriptional analysis was done on known abiotic (cold/freezing) regulated genes, where varietal differences were found. The knowledge attained from these endeavors is expected to expedite breeding of strawberries to achieve freezing stress tolerant lines.
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