Cristin-resultat-ID: 1524196
Sist endret: 7. desember 2017 13:07
Resultat
Vitenskapelig foredrag
2017

from light to dark and shallow to deep: Metagenomic analysis of total virus assemblages from arctic seawater provides a first glance of season- and depth-driven virus diversity and dynamics

Bidragsytere:
  • Jessica Louise Ray
  • François Enault
  • Torill Vik Johannessen
  • Gunnar Bratbak
  • Aud Larsen og
  • Ruth-Anne Sandaa

Konferanse

Tittel: 2017 Arctic Frontiers: White Space - Blue Future

Sted:

Tromsø
Norge
Utbredelsesområde: Internasjonalt
Årstall: 2017
Dato fra: 22. januar 2017
Dato til: 27. januar 2017

Arrangør:

Arctic Frontiers

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2017

Finansiering

  • Norges forskningsråd
    Prosjektkode: 268062
  • Norges forskningsråd
    Prosjektkode: 268489/O30

Beskrivelse Beskrivelse

Tittel

from light to dark and shallow to deep: Metagenomic analysis of total virus assemblages from arctic seawater provides a first glance of season- and depth-driven virus diversity and dynamics

Sammendrag

The enormous contrasts in light and seasonal productivity in high-latitude pelagic marine environments bespeaks an environment in which microorganisms must adapt to extremes in light and productivity. As important regulators of host diversity and nutrient recycling, viruses are critical components of the marine environment and the marine food web. The interdependence of viruses on the hosts they infect suggests that virus assemblages in high-latitude marine biomes may vary in step with season- and depth-driven variation in host populations. Furthermore, the extreme nature of the Arctic biome may house previously undescribed viral diversity. In order to survey of Arctic marine virus assemblages, we conducted research cruises to the west and north of Svalbard across one calendar year, sampling at depths from surface to 1000m. We extracted total nucleic acids from virus concentrates prepared from 50L of tangential flow-concentrated 0.45 or 0.2 µm-filtered seawater fractions. RNA and ssDNA virus genomes were converted to dsDNA using a combination of reverse transcription and second-strand synthesis. Total libraries, potentially representing all combinations of single-stranded or double-stranded and DNA or RNA virus genomes, were then amplified using random PCR and sequenced using Illumina MiSeq v3 PE300. Virus metagenomes were analyzed using both MG-RAST and by manual quality-trimming and assembly followed by analysis using the MetaVir2 platform. Preliminary taxonomic analysis of virus metagenomes suggests a high diversity of virus genome types, including a predominance of dsDNA and ssDNA genomes and a lesser contribution from RNA virus genomes. We observed large functional diversity in metagenomes, with an indication of depth-dependence on functional repertoire. Assembly of virus metagenomes resulted in the generation of almost entirely contigs

Bidragsytere

Jessica Louise Ray

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen

François Enault

  • Tilknyttet:
    Forfatter
    ved Université Blaise Pascal
  • Tilknyttet:
    Forfatter
    ved Centre national de la recherche scientifique

Torill Vik Johannessen

  • Tilknyttet:
    Forfatter
    ved Universitetet i Bergen

Gunnar Bratbak

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen

Aud Larsen

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen
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