Cristin-resultat-ID: 1617708
Sist endret: 19. mars 2019 11:29
NVI-rapporteringsår: 2018
Resultat
Vitenskapelig artikkel
2018

A genomic view of experimental intraspecies and interspecies transformation of a rifampicin-resistance allele into Neisseria meningitidis

Bidragsytere:
  • Kristian Alfsnes
  • Stephan Alfons Frye
  • Jens Eriksson
  • Vegard Eldholm
  • Ola Brønstad Brynildsrud
  • Jon Bohlin
  • mfl.

Tidsskrift

Microbial Genomics
ISSN 2057-5858
e-ISSN 2057-5858
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2018
Volum: 4
Hefte: 11
Sider: 1 - 14
Open Access

Importkilder

Scopus-ID: 2-s2.0-85057535538

Finansiering

  • Helse Sør-Øst RHF
    Prosjektkode: 2014050
  • Helse Sør-Øst RHF
    Prosjektkode: 2017110
  • Norges forskningsråd
    Prosjektkode: 142035
  • Norges forskningsråd
    Prosjektkode: 196394
  • Norges forskningsråd
    Prosjektkode: 220901
  • Norges forskningsråd
    Prosjektkode: 261669
  • Norges forskningsråd
    Prosjektkode: 271874

Beskrivelse Beskrivelse

Tittel

A genomic view of experimental intraspecies and interspecies transformation of a rifampicin-resistance allele into Neisseria meningitidis

Sammendrag

The spread of antibiotic resistance within and between different bacterial populations is a major health problem on a global scale. The identification of genetic transformation in genomic data from Neisseria meningitidis, the meningococcus (Mc), and other bacteria is problematic, since similar or even identical alleles may be involved. A particular challenge in naturally transformable bacteria generally is to distinguish between common ancestry and true recombined sites in sampled genome sequences. Furthermore, the identification of recombination following experimental transformation of homologous alleles requires identifiable differences between donor and recipient, which in itself influences the propensity for homologous recombination (HR). This study identifies the distribution of HR events following intraspecies and interspecies Mc transformations of rpoB alleles encoding rifampicin resistance by whole-genome DNA sequencing and single nucleotide variant analysis. The HR events analysed were confined to the genomic region surrounding the single nucleotide genetic marker used for selection. An exponential length distribution of these recombined events was found, ranging from a few nucleotides to about 72 kb stretches. The lengths of imported sequences were on average found to be longer following experimental transformation of the recipient with genomic DNA from an intraspecies versus an interspecies donor (P

Bidragsytere

Kristian Alfsnes

  • Tilknyttet:
    Forfatter
    ved Avdeling for molekylærbiologi ved Folkehelseinstituttet

Stephan Alfons Frye

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF

Jens Eriksson

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF

Vegard Eldholm

  • Tilknyttet:
    Forfatter
    ved Avdeling for molekylærbiologi ved Folkehelseinstituttet
Aktiv cristin-person

Ola Brønstad Brynildsrud

  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsepidemiologi og modellering ved Folkehelseinstituttet
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