Cristin-resultat-ID: 1765574
Sist endret: 7. februar 2020 10:59
NVI-rapporteringsår: 2019
Resultat
Vitenskapelig artikkel
2019

Lipid and DHA-production in Aurantiochytrium sp. – Responses to nitrogen starvation and oxygen limitation revealed by analyses of production kinetics and global transcriptomes

Bidragsytere:
  • Tonje Marita Bjerkan Heggeset
  • Helga Ertesvåg
  • Bin Liu
  • Trond Erling Ellingsen
  • Olav Vadstein og
  • Inga Marie Aasen

Tidsskrift

Scientific Reports
ISSN 2045-2322
e-ISSN 2045-2322
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2019
Publisert online: 2019
Volum: 9
Sider: 1 - 13
Artikkelnummer: 19470
Open Access

Importkilder

Scopus-ID: 2-s2.0-85076917208

Klassifisering

Vitenskapsdisipliner

Bioteknologi

Beskrivelse Beskrivelse

Tittel

Lipid and DHA-production in Aurantiochytrium sp. – Responses to nitrogen starvation and oxygen limitation revealed by analyses of production kinetics and global transcriptomes

Sammendrag

Thraustochytrids of the genera Schizochytrium and Aurantiochytrium accumulate oils rich in the essential, marine n3 fatty acid docosahexaenoic acid (DHA). DHA production in Aurantiochytrium sp T66 was studied with the aim to provide more knowledge about factors that affect the DHA-productivities and the contributions of the two enzyme systems used for fatty acid synthesis in thraustochytrids, fatty acid synthetase (FAS) and PUFA-synthase. Fermentations with nitrogen starvation, which is well-known to initiate lipid accumulation in oleaginous organisms, were compared to fermentations with nitrogen in excess, obtained by oxygen limitation. The specific productivities of fatty acids originating from FAS were considerably higher under nitrogen starvation than with nitrogen in excess, while the specific productivities of DHA were the same at both conditions. Global transcriptome analysis showed significant up-regulation of FAS under N-deficient conditions, while the PUFA-synthase genes were only marginally upregulated. Neither of them was upregulated under O2-limitation where nitrogen was in excess, suggesting that N-starvation mainly affects the FAS and may be less important for the PUFA-synthase. The transcriptome analysis also revealed responses likely to be related to the generation of reducing power (NADPH) for fatty acid synthesis.

Bidragsytere

Aktiv cristin-person

Tonje Marita Bjerkan Heggeset

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Helga Ertesvåg

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet

Bin Liu

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet

Trond Erling Ellingsen

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Olav Vadstein

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet
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