Miniaturizing LC-MS for high sensitivity clinical sample analyses

Clinical samples often exhibit limited sample sizes and analytes being present below the detection limit of the method, thus hampering the analytical assessment and consequently the research outcome. By narrowing the inner diameter of the separation column, the sample dilution is vastly reduced, adding to sensitivity. Hence in this study, miniaturized LC-MS has been widely used, allowing for sensitive proteomic measurements in limited samples from cell derived nanosized exosomes, patient derived glioblastoma cells and stem cell derived 3D organ models (i.e. organoids), subsequently providing with fundamental information on disease development, characteristics and responses on therapeutics.