Cristin-resultat-ID: 1810918
Sist endret: 18. februar 2021, 13:30
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles

Bidragsytere:
  • Leila Allahgholi
  • Roya R.R. Sardari
  • Sigrid Hakvåg
  • Kazi Z.G. Ara
  • Thordis Anna Kristjansdottir
  • Inga Marie Aasen
  • mfl.

Tidsskrift

Journal of Applied Phycology
ISSN 0921-8971
e-ISSN 1573-5176
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Publisert online: 2020
Trykket: 2020
Volum: 32
Sider: 1933 - 1947
Open Access

Importkilder

Scopus-ID: 2-s2.0-85083117460

Beskrivelse Beskrivelse

Tittel

Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles

Sammendrag

Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems need to be solved to make them a viable option. Pre-treatment is necessary to enhance accessibility and solubility of the biomass components but should be minimal and mild to assure sustainable and cost-effective processing. Here, two routes to pre-treatLaminaria digitata to release polysaccharides were investigated: hot water pre-treatment by autoclaving (121 °C, 20 min or 60 min) and a two-step extraction with mild acid (0.1 M HCl) followed by alkaline treatment. Hot water pre-treatment resulted in partial extraction of a mixture of polysaccharides consisting of alginate, fucoidan and laminarin. After mild acid pre-treatment, alginate was found in the remaining insoluble residues and was extracted in a second step via alkaline treatment using Na2CO3 (0.15 M) at 80 °C and CaCl2 (10%) for the precipitation. In addition to carbohydrates, a fraction of other components such as proteins, phenolic compounds, minerals and trace elements was detected in the extracts. Cultivation of the thermophilic bacterial strains Rhodothermus marinus DSM 16675 and Bacillus methanolicus MGA3 (ATCC 53907) in media supplemented with the respective extracts resulted in growth of both strains, indicating that they were able to utilize the available carbon source for growth. R. marinus displayed the highest cell density in the medium containing the extract from acid pre-treatment, whereas B. methanolicus growth was highest with the extract from hot water pre-treatment.

Bidragsytere

Leila Allahgholi

  • Tilknyttet:
    Forfatter
    ved Lunds universitet

Roya Sardari

Bidragsyterens navn vises på dette resultatet som Roya R.R. Sardari
  • Tilknyttet:
    Forfatter
    ved Lunds universitet

Sigrid Hakvåg

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet

Kazi Ara

Bidragsyterens navn vises på dette resultatet som Kazi Z.G. Ara
  • Tilknyttet:
    Forfatter
    ved Lunds universitet

Thordis Anna Kristjansdottir

  • Tilknyttet:
    Forfatter
    ved Matís
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