Pioneering the Details: A Multi-Method Collaboration to Gain Insight Into Structure and Thermal Stability of Lipid Nanoparticles (LNPs) for mRNA Delivery

Characterization of stability and structure of vectors for RNA/DNA delivery is of increasing importance. Here, a combination of orthogonal biophysical techniques of differential scanning calorimetry, DSC, and circular dichroism, CD, was used to evaluate and compare Higher Order Structure and thermal stability of mRNA and LNPs w/wo mRNA payload. Evidence collected in this study indicates that mRNA-LNP assemblies are stabilized by interactions against thermal-induced transitions and disintegration.