Cristin-resultat-ID: 370099
Sist endret: 2. juni 2017, 12:07
NVI-rapporteringsår: 2007
Resultat
Vitenskapelig artikkel
2007

Extraction of microalgal toxins by large-scale pumping of seawater in Spain and Norway, and isolation of okadaic acid and dinophysistoxin-2

Bidragsytere:
  • Thomas Rundberget
  • Morten Sandvik
  • Kristofer Larsen
  • G.M. Pizarro
  • Beatriz Reguera
  • T Castberg
  • mfl.

Tidsskrift

Toxicon
ISSN 0041-0101
e-ISSN 1879-3150
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2007
Volum: 50
Hefte: 7
Sider: 960 - 970

Importkilder

Scopus-ID: 2-s2.0-35648965736
Isi-ID: 000251476400009

Klassifisering

Vitenskapsdisipliner

Marinbiologi

Beskrivelse Beskrivelse

Tittel

Extraction of microalgal toxins by large-scale pumping of seawater in Spain and Norway, and isolation of okadaic acid and dinophysistoxin-2

Sammendrag

Marine biotoxins from microalgae can accumulate in shellfish and lead to poisoning of human consumers as well as fish, marine mammals and sea birds. Toxicological assessment of the toxins and development of analytical methods require large amounts of high-purity toxins and their metabolites. Although these toxins can be obtained in limited amounts from contaminated shellfish or from microalgal cultures, difficulties arise when the toxin-producing microalga is difficult to culture or its identity is not known. To circumvent this problem, we have developed a large-scale method for solid-phase extraction (SPE) of lipophilic biotoxins from natural microalgal blooms in seawater. To enhance subsequent purification of toxins adsorbed on the column, we included a filtration step to release the toxins from the cells while removing insoluble compounds and cellular debris. The efficacy of the method was illustrated by extraction and purification of okadaic acid and dinophysistoxin-2 from a high-density Dinophysis acuta bloom in Spain and from a mixed bloom containing low densities of D. acuta in Norway. Isolation of the toxins adsorbed on the SPE column was simple and efficient, and results obtained so far indicate that the method is potentially applicable to a wide range of microalgal toxins such as azaspiracids, pectenotoxins, spirolides and microcystins. The method should also be useful for harvesting toxins from large-scale microalgal cultures, and for bioprospecting for and isolation of bioactive natural products from marine and freshwater environments.

Bidragsytere

Jan Thomas Rundberget

Bidragsyterens navn vises på dette resultatet som Thomas Rundberget
  • Tilknyttet:
    Forfatter

Morten Sandvik

  • Tilknyttet:
    Forfatter

Kristofer Larsen

  • Tilknyttet:
    Forfatter
    ved Kjemisk institutt ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Norges miljø- og biovitenskapelige universitet

G.M. Pizarro

  • Tilknyttet:
    Forfatter

Beatriz Reguera

  • Tilknyttet:
    Forfatter
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