Sammendrag
Using the European common frog, Rana temporaria, as a model, we have studied the organ-specific gene expression patterns of thyroid hormone receptor isoforms after exposure to an organochlorine (OC) compound, p,p´-DDE. Four groups of frogs were subcutaneously injected with p,p�-DDE at 0.01, 0.1, 1 and 10 mg/kg body weight, respectively. In addition, one group, serving as the control group, was injected with pure corn oil. TH receptor isoforms (TRa and TRb) gene expressions were evaluated in the brain, kidney, testis and liver using real-time PCR with gene-specific primers. Our results show that p,p�-DDE doses induced slight elevations of TRa and TRb mRNA in the brain. In the testis, p,p�-DDE induced an initial significant 3-fold increase of TRa mRNA at 0.01 mg/kg and thereafter clear dose-dependent decreases of TRa mRNA levels were observed. For testicular TRb mRNA levels, p,p�-DDE induced a slight elevation at 0.01 mg/kg and thereafter significant decreases in TRb mRNA levels were observed. p,p�-DDE induced significant 2-4-fold elevations of both TR isoforms in frog kidney. The strongest transcriptional effect of p,p�-DDE on TR isoforms was observed in the kidney. While TRa mRNA was not measurable in the liver, p,p�-DDE induced an initial 1.7-fold increase at 0.01 mg/kg of TRb mRNA and thereafter an apparent dose-dependent decrease was observed. The relative abundance of TRa and TRb gene expression in different organs are in the order: kidney>testis>brain>liver. While the induction TRa and TRb might result to hypersensitivity and subsequent gain of biological functions, the inhibition might result to loss of biological function. Given the high persistency in the environment and continued use in developing countries coupled with the tendency for global atmospheric transport, DDT and its metabolites such as p,p´-DDE will remain a focus of concern both for scientific and societal reasons.
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