Sammendrag
Gametophore fragments from two populations of each of the closely related Sphagnum fallax and S. isoviitae were regenerated on agar in axenic culture. Fragments from stem branches and capitulum branches were grown in media with or without additions of glucose and cytokinin in order to find conditions for rapid production of a large number of clonal gametophores. The morphology of the regenerated material was compared with the field collected gametophores. After 5 weeks 62 % of the cultures had regenerated. The frequency of fragments regenerating was considerably higher from capitulum branch fragments than stem branch fragments, while cytokinin seemed to hamper both initiation of regeneration and growth of the regenerated material. Addition of glucose caused extensive losses of cultures due to contamination. The regenerated material was distinctly different morphologically from the field collected gametophores in having larger branch leaves and large hemi-isophyllous stem leaves. Gametophores initiated from stem branch fragments had larger stem leaves than those initiated from capitulum branches. Morphological characters varied considerably between cultures of the same genotype, but much less within clonal cultures suggesting that cultures are in different stages of development. The experiment shows that regeneration on agar in axenic culture is an adequate method for fast propagation of clonal material for a diversity of purposes, and su- ggests ways of optimising this approach.
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