The Study on the Properties of Self-Assembled Bimolecular Layers at the Gold Electrode with Incorporated Calixarenes for Dopamine and Epinephrine Detection

Supported self-assembled lipid films (sBLM) on a gold electrode have been used as support for calix[4]arene (C1) and calix[6]arene (C2). The biosensing ability of such structures for dopamine (DA) and epinephrine (EP), was studied by electrochemical impedance spectroscopy (EIS). We showed that calixarenes selectively discriminate DA and EP. The sensitivity of detection by means of capacitance changes of EP was higher for C1 in comparison with C2 and reached 30 μmol⋅L−1. DA has been detected with much lower sensitivity (approx. 100 μM) that did not depend on the type of calixarene used. However, sensitivity of detection of DA by means of charge transfer resistance, Rct, determined from impedance spectra was much higher, reaching 1.0 μmol⋅L−1.