Sammendrag
Rates of glucose, amino acid and dipeptide absorption by the intestine of Atlantic salmon (Salmo salar L.) were measured in vitro at 10°C as functions of concentration and region using intact tissues (everted sleeve method). Salmon (weight range 300-1300 g) fed a commercial, extruded salmon feed, were kept at 12-13°C in freshwater. Maximum rates (Vmax) of glucose and dipeptide transport were low compared to most amino acid Vmax values. There was a declining proximal-to-distal gradient of absorption along the post-gastric intestinal tract. A saturable component of absorption was not evident for proline and glycyl-proline in the distal intestine, and glycyl-sarcosine in any region. "Apparent diffusion", which may include low affinity, high capacity carrier systems and carrier-independent influx, appears to account for the majority of total uptake at higher concentrations of amino acids and dipeptides. There was competition between the dipeptides for transporter sites in the pyloric ceca and mid intestine, suggesting a common carrier. There was also indication of hydrolysis of these dipeptides by brush border membrane enzymes.
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