Sammendrag
Scarce resources of mineral oils and environmental issues generate an interest in renewable fuel sources. Fish oil could be used as a source for biodiesel in countries with plenty of fishery and fish farming to satisfy the energy demand partly. Lipases significantly decrease the negative environmental impact of conventional biodiesel production since waste water treatment is no longer required, as one example. In this report cod liver oil was used for the enzymatic production of biodiesel in a solvent free assay utilizing porcine pancreas lipase (PPL) and lipases from Candida antarctica and Thermomyces lanuginosa. The influence of different amounts of methanol in the reaction mixture was examined and a stepwise addition of the alcohol to the reaction mixture was compared to a system with initial addition of the whole amount of methanol. The highest yield obtained was with C. antarctica (71 %), incubating 2 mmol cod liver oil with 6 mmol methanol, added stepwise, and with T. lanuginosa (67 %) which was incubated with 2 mmol cod liver oil and 8 mmol methanol. Both were incubated for 24 h, at 37 °C, and shaking at 150 rpm. Due to the high price of enzymatic catalysts, lipases are not economically competitive to conventional chemical synthesis of Biodiesel. Therefore three attempts were made to isolate lipases from Atlantic salmon (Salmo salar) liver, a waste product of fish farming which could serve as a cheap, easily available lipase source. The methods were chosen with regards to their suitability for industrial application.
Vis fullstendig beskrivelse
