Sammendrag
Abstract The aim of this study was to investigate the dissociation of the fatty acid residues from human blood glycerophospholipids with electrospray tandem quadrupole mass spectrometry. Early observations gave some indications that the main fragmentation pathways were collison energy dependent. The classes investigated were phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidic acid (PA) containing endogenous C18:0 (sn-1 position) and C20:4 (sn-2 position) fatty acyl residues. The experiments were done using normal-phase liquid chromatography coupled to negative ion electrospray tandem mass spectrometry. The operation mode was multiple reaction monitoring (MRM) of the transitions from M-H- m/z=283.3 (C18:0) and from M-H- m/z=303.2 (C20:4). The resulting peak areas for the two transitions for each class were expressed as the ratio R: R= m/z=283.3/m/z=303.3 The ratio R was found to increase with increasing collision energy for all classes and for a g
Vis fullstendig beskrivelse
