Cristin-resultat-ID: 916794
Sist endret: 30. oktober 2017, 10:59
NVI-rapporteringsår: 2012
Resultat
Vitenskapelig artikkel
2012

Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.)

Bidragsytere:
  • Jareeporn Ruangsri
  • Scott Salger
  • Christopher Caipang
  • Kiron Viswanath og
  • Jorge Fernandes

Tidsskrift

Fish and Shellfish Immunology
ISSN 1050-4648
e-ISSN 1095-9947
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2012
Volum: 32
Hefte: 3
Sider: 396 - 406

Importkilder

Isi-ID: 000300817300003
Scopus-ID: 2-s2.0-84855958149

Beskrivelse Beskrivelse

Tittel

Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.)

Sammendrag

The piscidin (pis) family of potent antimicrobial peptides with broad-spectrum activity has an important role in innate host defence. We have identified and characterized two pis paralogues in Atlantic cod (pis1 and pis2), as well as a novel splice variant of pis2, termed pis2-β. Pis1 and pis2 genes have most likely originated from a recent duplication event, since they share the same four-exon structure with up to 91% identity at the intron level. The alternative transcript pis2-β is derived from intron retention and even if not translated it may regulate pis expression through nonsense mediated decay. In spite of their overall conservation, pis genes are being shaped by positive selection and pis1, pis2 and pis2-β code for structurally diverse mature peptides, which have different functional properties. Synthetic Pis1 displays antibacterial activity in the micromolar range against Gram-(+) and Gram-(-) bacteria, including the fish pathogens Vibrio anguillarum and Yersinia ruckeri. In contrast, synthetic Pis2 and Pis2-β have limited or no antibacterial activity, respectively, but exhibit more potent antiparasitic activity against Tetrahymena pyriformis. In adult cod, pis1 and pis2-β are constitutively expressed in immune-related organs, whereas pis2 is constitutively expressed in all tissues examined. Differential expression is also observed during embryonic development. In particular, pis2 and pis2-β are maternally inherited but pis1 transcripts are only present from gastrulation onwards. It was found that antigenic challenge with attenuated V. anguillarum induces a general down-regulation of all pis in head kidney, spleen and distal intestine, suggesting that they may be used as health indicators. Taken together, our data indicate that pis is an important component of the cod innate immune system. Moreover, the two pis paralogues have undergone structural diversification and it is likely that they play multifunctional roles in Atlantic cod.

Bidragsytere

Ruangsri Jareeporn

Bidragsyterens navn vises på dette resultatet som Jareeporn Ruangsri
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Scott Salger

  • Tilknyttet:
    Forfatter
    ved North Carolina State University

Christopher Caipang

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Kiron Viswanath

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

Bidragsyterens navn vises på dette resultatet som Jorge Fernandes
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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